In vivo evaluation of the biocompatibility of surface modified hemodialysis polysulfone hollow fibers in rat
| Autor: | Siddharth S. Sabale, Laxman B. Sarkate, Sachin Kadam, Ganpat J. Dahe, Jayesh R. Bellare, Dattatray P. Kadam |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Drugs and Devices Histology Biocompatibility Angiogenesis Polymers Surface Properties Materials Science lcsh:Medicine Biocompatible Materials Polyethylene glycol Biochemistry Biomaterials Medical Devices chemistry.chemical_compound Model Organisms Implants Experimental In vivo Renal Dialysis Materials Testing medicine Animals Polysulfone Sulfones Fibroblast lcsh:Science Biology Multidisciplinary Phosphorylcholine lcsh:R Membranes Artificial Animal Models Hematology Surgery Rats Membrane medicine.anatomical_structure chemistry Nephrology Cytochemistry Rat Medicine lcsh:Q Dialysis Immunocytochemistry Biomedical engineering Research Article |
| Zdroj: | PLoS ONE, Vol 6, Iss 10, p e25236 (2011) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Polysulfone (Psf) hollow fiber membranes (HFMs) have been widely used in blood purification but their biocompatibility remains a concern. To enhance their biocompatibility, Psf/TPGS (d-α-tocopheryl polyethylene glycol 1000 succinate) composite HFMs and 2-methacryloyloxyethyl phosphorylcholine (MPC) coated Psf HFMs have been prepared. They have been evaluated for in vivo biocompatibility and graft acceptance and compared with sham and commercial membranes by intra-peritoneal implantation in rats at day 7 and 21. Normal body weights, tissue formation and angiogenesis indicate acceptance of implants by the animals. Hematological observations show presence of post-surgical stress which subsides over time. Serum biochemistry results reveal normal organ function and elevated liver ALP levels at day 21. Histological studies exhibit fibroblast recruitment cells, angiogenesis and collagen deposition at the implant surface indicating new tissue formation. Immuno-histochemistry studies show non-activation of MHC molecules signifying biocompatibilty. Additionally, Psf/TPGS exhibit most favorable tissue response as compared with other HFMs making them the material of choice for HFM preparation for hemodialysis applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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