Serial expression of the truncated fragments of the nucleocapsid protein of CCHFV and identification of the epitope region
| Autor: | Fei Deng, Yan-jun Luo, Hualin Wang, Yujiang Zhang, Fuchun Zhang, Surong Sun, Zhihong Hu, Peng-fei Wei, Tianxian Li |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Blotting
Western Immunology Gene Expression Antibodies Viral Article Epitope Virus law.invention Epitopes law Virology Gene expression Escherichia coli Animals Gene Sequence Deletion Expression vector biology Antibodies Monoclonal Nucleocapsid Proteins Molecular biology Recombinant Proteins Polyclonal antibodies Hemorrhagic Fever Virus Crimean-Congo biology.protein Recombinant DNA Molecular Medicine Mutant Proteins Rabbits Antibody Epitope Mapping |
| Zdroj: | Virol Sin |
| ISSN: | 1995-820X 1674-0769 |
| DOI: | 10.1007/s12250-010-3067-7 |
| Popis: | The Crimean-congo hemorrhagic fever virus (CCHFV) is a geographically widespread fatal pathogen. Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections. In this study, expression vectors carrying series truncated fragments of the NP (nucleocapsid protein) gene from the S fragment of CCHFV strain YL04057 were constructed. The recombinant proteins were expressed in E.coli and purified for detection. The antigenic of the truncated fragments of NP was detected with a polyclonal serum (rabbit) and 2 monoclonal (mAbs) (14B7 and 43E5) against CCHFV by Western-blot analyses. The results showed that the three expressed constructs, which all contained the region 235AA to 305AA could be detected by mAbs polyclonal serum. The results suggest that region 235-305 aa of NP is a highly antigenic region and is highly conserved in the NP protein. |
| Databáze: | OpenAIRE |
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