High-protein diet more effectively reduces hepatic fat than low-protein diet despite lower autophagy and FGF21 levels

Autor:	Stefan Kabisch, Anne Loft, Mario Ost, Jennifer Loske, Chenchen Xu, Susanne Klaus, Mariya Markova, Thomas Gantert, Anke Rosenthal, Olga Pivovarova-Ramich, Volker Lange, Kathleen Herz, Tilman Grune, Andreas Pfeiffer, Stephan Herzig, Frederick Klauschen, Silke Hornemann, Nicole Seebeck, Verena Coleman, Jürgen Machann
Rok vydání:	2020
Předmět:	medicine.medical_specialty Low protein FGF21 medicine.medical_treatment High-protein diet medicine.disease_cause Diet High-Fat 03 medical and health sciences 0302 clinical medicine Low-protein diet Weight loss Internal medicine Lipid biosynthesis medicine Autophagy Diet Protein-Restricted Humans Dietary Protein Er-stress Fgf21 Liver Fat Mitochondria Nutrition Obesity Hepatology business.industry Fatty liver medicine.disease Diet Fibroblast Growth Factors Endocrinology Liver 030220 oncology & carcinogenesis Diet High-Protein 030211 gastroenterology & hepatology Dietary Proteins medicine.symptom business
Zdroj:	Liver Int. 40, 2982-2997 (2020)
ISSN:	1478-3231
Popis:	Background and aims Non-alcoholic fatty liver disease (NAFLD) is becoming increasingly prevalent and nutrition intervention remains the most important therapeutic approach for NAFLD. Our aim was to investigate whether low- (LP) or high-protein (HP) diets are more effective in reducing liver fat and reversing NAFLD and which mechanisms are involved. Methods 19 participants with morbid obesity undergoing bariatric surgery were randomized into two hypocaloric (1500-1600 kcal/day) diet groups, a low protein (10E% protein) and a high protein (30E% protein), for three weeks prior to surgery. Intrahepatic lipid levels (IHL) and serum fibroblast growth factor 21 (FGF21) were measured before and after the dietary intervention. Autophagy flux, histology, mitochondrial activity, and gene expression analyses were performed in liver samples collected during surgery. Results IHL levels decreased by 42.6% in the HP group, but were not significantly changed in the LP group despite similar weight loss. Hepatic autophagy flux and serum FGF21 increased by 66.7% and 42.2%, respectively, after 3 weeks in the LP group only. Expression levels of fat uptake and lipid biosynthesis genes were lower in the HP group compared with those in the LP group. RNA-seq analysis revealed lower activity of inflammatory pathways upon HP diet. Hepatic mitochondrial activity and expression of β-oxidation genes did not increase in the HP group. Conclusions HP diet more effectively reduces hepatic fat than LP diet despite of lower autophagy and FGF21. Our data suggest that liver fat reduction upon HP diets result primarily from suppression of fat uptake and lipid biosynthesis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b1dfb8ffd0cc6b2a5074896f1d32b8a https://pubmed.ncbi.nlm.nih.gov/32652799 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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