A Sphingomyelin-transferring Protein from Chicken Liver. USE OF PYRENE-LABELED PHOSPHOLIPID
| Autor: | Karel W. A. Wirtz, Gerry T. Snoek, Klaas-Jan de Vries, Jan Westerman, Pentti Somerharju, Johanna L.P.M. Timmermans-Hereijgers |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Gene isoform
Molecular Sequence Data Phospholipid Biology Biochemistry 03 medical and health sciences chemistry.chemical_compound Western blot Phospholipid transfer protein Phosphatidylcholine Pi medicine Animals Amino Acid Sequence Phosphatidylinositol Phospholipid Transfer Proteins Molecular Biology Phospholipids 030304 developmental biology 0303 health sciences Binding Sites Pyrenes Sequence Homology Amino Acid medicine.diagnostic_test 030302 biochemistry & molecular biology Membrane Proteins Cell Biology Molecular biology Rats Sphingomyelins 3. Good health Liver chemistry lipids (amino acids peptides and proteins) Carrier Proteins Sphingomyelin Chickens |
| Zdroj: | Journal of Biological Chemistry. 270:14263-14266 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.270.24.14263 |
| Popis: | A phospholipid transfer protein was purified from chicken liver which, in addition to phosphatidylinositol (PI) and phosphatidylcholine (PC), carries sphingomyelin (SM) between membranes. For comparison, the PI-transfer protein from chicken liver only carries PI and PC. Specificity was established by use of phospholipids that carry a pyrene-labeled acyl chain. Based on the N-terminal sequence and Western blot analysis we conclude that this protein is an isoform of the PI-transfer protein. At increasing length of the pyrene-labeled acyl chain, the isoform expresses a high activity toward SM, a low activity toward PI, and virtually no activity toward PC. |
| Databáze: | OpenAIRE |
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