Rapid cellular enrichment of eicosapentaenoate after a single intravenous injection of a novel medium-chain triacylglycerol:fish-oil emulsion in humans
| Autor: | Isabelle E. Dupont, Mirjam Hacquebard, Laurence Portois, Yvon Carpentier, Willy Malaisse, Richard J. Deckelbaum |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
Blood Platelets Male medicine.medical_specialty Fat Emulsions Intravenous medicine.medical_treatment alpha-Tocopherol Medicine (miscellaneous) Fatty Acids Nonesterified chemistry.chemical_compound Bolus (medicine) Fish Oils Double-Blind Method Internal medicine medicine Leukocytes Humans Phospholipids Triglycerides chemistry.chemical_classification Nutrition and Dietetics Chromatography Cross-Over Studies Fatty Acids Essential Vitamin E Fatty acid food and beverages Fish oil Eicosapentaenoic acid Dietary Fats Lipids Endocrinology chemistry Blood chemistry Eicosapentaenoic Acid Injections Intravenous lipids (amino acids peptides and proteins) Polyunsaturated fatty acid |
| Zdroj: | The American journal of clinical nutrition. 91(4) |
| ISSN: | 1938-3207 |
| Popis: | Background: Dietary deficiency in n23 (omega-3) polyunsaturated fatty acids (PUFAs) prevails in Western populations and potentially results in adverse health outcomes. To circumvent the slow n23 PUFA incorporation in phospholipids of key cells after oral supplementation, a new preparation for intravenous bolus injection was developed with 20 g triacylglycerols/100 mL of a mixture of 80% medium-chain triacylglycerols (MCTs) and 20% fish oil (FO) (wt:wt), and 0.4 g a-tocopherol/100 mL of the same mixture. Objective: Our objective was to document the enrichment of n23 PUFAs in leukocyte and platelet phospholipids after a bolus intravenous injection of MCT:FO in men. Design: Twelve healthy male subjects received injections over a 5-min period of 50 mL of either MCT:FO or a control MCT:long-chain triacylglycerol (MCT:LCT) emulsion containing 20 g triacylglycerols/100 mL with equal amounts (wt:wt) of MCT and soybean triacylglycerols (LCT) and containing 0.02 g a-tocopherol/100 mL; after an 8-wk interval, the subjects received injections of the other preparation. Results: Clinical and biological variables that assessed tolerance and safety remained unchanged. Plasma elimination was faster for MCT:FO than for MCT:LCT (half-life: 24.5 6 3.5 min compared with 32.9 6 3.0 min; P , 0.025). This was associated with a greater increase in the plasma nonesterified fatty acid concentration. The content of n23 PUFAs, specifically eicosapentaenoic acid (20:5n23), increased in leukocyte and platelet phospholipids within 60 min and 24 h after MCT:FO injection. Conclusion: Bolus intravenous injection of a novel MCT:FO emulsion allows rapid enrichment of cells with n23 PUFAs. Am J Clin Nutr 2010;91:875‐82. |
| Databáze: | OpenAIRE |
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