System-wide Clinical Proteomics of Breast Cancer Reveals Global Remodeling of Tissue Homeostasis
| Autor: | Iris Barshack, Cecilia Lindskog, Tamar Geiger, Nora Balint-Lahat, Rotem Katzir, Fredrik Pontén, Jan Daniel Rudolph, Camilla Avivi, Yair Pozniak, Eytan Ruppin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
CA15-3 Histology Cancer Cell Biology Biology medicine.disease Bioinformatics medicine.disease_cause Proteomics Pathology and Forensic Medicine 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Breast cancer Cancer cell medicine Cancer research Carcinogenesis Lymph node Tissue homeostasis |
| Zdroj: | Cell Systems. 2(3):172-184 |
| ISSN: | 2405-4712 |
| DOI: | 10.1016/j.cels.2016.02.001 |
| Popis: | The genomic and transcriptomic landscapes of breast cancer have been extensively studied, but the proteomes of breast tumors are far less characterized. Here, we use high-resolution, high-accuracy mass spectrometry to perform a deep analysis of luminal-type breast cancer progression using clinical breast samples from primary tumors, matched lymph node metastases, and healthy breast epithelia. We used a super-SILAC mix to quantify over 10,000 proteins with high accuracy, enabling us to identify key proteins and pathways associated with tumorigenesis and metastatic spread. We found high expression levels of proteins associated with protein synthesis and degradation in cancer tissues, accompanied by metabolic alterations that may facilitate energy production in cancer cells within their natural environment. In addition, we found proteomic differences between breast cancer stages and minor differences between primary tumors and their matched lymph node metastases. These results highlight the potential of proteomic technology in the elucidation of clinically relevant cancer signatures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|