Structure and rheological properties of a xyloglucan extracted from Hymenaea courbaril var. courbaril seeds
| Autor: | Paulo A.S. Mourão, Maria G. Carneiro-da-Cunha, António A. Vicente, Maria Tereza dos Santos Correia, Isabel R.S. Arruda, Gustavo R.C. Santos, Alexandre Gomes da Silva, Priscilla B.S. Albuquerque |
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| Přispěvatelé: | Universidade do Minho |
| Jazyk: | angličtina |
| Předmět: |
Arabinose
Polysaccharide structure 02 engineering and technology Hymenaea courbaril Xylose Polysaccharide Biochemistry Methylation Polysaccharide characterization 03 medical and health sciences chemistry.chemical_compound Viscoelastic fluid Dynamic light scattering X-Ray Diffraction Structural Biology Xyloglucan Molecular Biology Glucans Nuclear Magnetic Resonance Biomolecular Ethanol precipitation 030304 developmental biology chemistry.chemical_classification 0303 health sciences Science & Technology Chromatography biology Molecular Structure Hemolytic Agents Plant Extracts General Medicine 021001 nanoscience & nanotechnology biology.organism_classification 3. Good health Anti-Bacterial Agents chemistry Galactose Seeds Chromatography Gel Xylans Hymenaea 0210 nano-technology Rheology |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2014.11.001 |
| Popis: | Available online 18 November 2014 Hymenaea courbaril var courbaril seed xyloglucan was efficiently extracted with 0.1 M NaCl, followed by ethanol precipitation (yield = 72 ± 5% w/w). Its amorphous structure was identified by the pattern of X-ray diffraction. The monosaccharide composition was determined by GC/MS analysis of the alditol acetates and showed the occurrence of glucose:xylose:galactose:arabinose (40:34:20:6). One-(1D) and two-dimensional-(2D) NMR spectra confirmed a central backbone composed by 4-linked β-glucose units partially branched at position 6 with non-reducing terminal units of α-xylose or β-galactose-(1→2)-α-xylose disaccharides. The xyloglucan solution was evaluated by dynamic light scattering and presents a polydisperse and practically neutral profile, and at 0.5 and 1.0% (w/v) the solutions behave as a viscoelastic fluid. The polysaccharide did not show significant antibacterial or hemolytic activities. Overall our results indicate that xyloglucan from H. courbaril is a promising polysaccharide for food and pharmaceutical industries. The authors Isabel R.S. Arruda, Priscilla B.S. de Albuquerque and Alexandre G. Silva are recipient of a scholarship from Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE). Gustavo R.C. Santos is recipient of a scholarship from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq). The authors express their gratitude to the CNPq for research grants and fellowship (MTSC, PASM, MGCC). The authors acknowledge Centro de Tecnologias Estrategicas do Nordeste (CETENE) by analitical support and CNPq and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) by financial support. Author Antonio A. Vicente wishes to thank the Fundacao para a Ciencia e a Tecnologia (FCT) Strategic Project PEst-OE/EQB/LA0023/2013 and the Project "BioInd - Biotechnology and Bioengineering for improved Industrial and Agro-Food processes", REF. NORTE-07-0124-FEDER-000028, co-funded by the Programa Operacional Regional do Norte (ON.2-O Novo Norte), QREN and FEDER (Portugal). |
| Databáze: | OpenAIRE |
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