Phanerochaete chrysosporium as a model organism to assess the toxicity of municipal landfill leachate from Elazığ, Turkey
| Autor: | Numan Yildirim, Sule Tatar, Hevidar Alp, Nuran Cikcikoglu Yildirim |
|---|---|
| Přispěvatelé: | Tunceli Meslek Yüksekokulu, Mühendislik Fakültesi |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Antioxidant
Turkey Health Toxicology and Mutagenesis medicine.medical_treatment 010501 environmental sciences Ecotoxicology Phanerochaete 01 natural sciences Antioxidants Superoxide dismutase chemistry.chemical_compound medicine Environmental Chemistry Leachate Food science 0105 earth and related environmental sciences Chrysosporium Biological Oxygen Demand Analysis biology Superoxide Dismutase Chemistry Landfill leachate Phanerochaete chrysosporium Biochemical response General Medicine Glutathione Hydrogen-Ion Concentration Catalase biology.organism_classification Total dissolved solids Pollution Oxidative Stress biology.protein Oxidation-Reduction Water Pollutants Chemical |
| Popis: | In order to evaluate the potential ecological risk and the toxic effect of landfill leachate (LL), Phanerochaete chrysosporium was exposed to LL and their biochemical response was observed by using antioxidant parameters. Phanerochaete chrysosporium, ME 446, was kept at 4 °C after being sub-cultured at 28 °C on Sabouraud Dextrose Agar (SDA). Superoxide dismutase (SOD), catalase (CAT) activities, and malaondialdehyde (MDA) and glutathione (GSH) levels of P. chrysosporium exposed to different dilution rates of leachate (1/10 and 1/20) for 24 and 96 h were analyzed by using the ELISA method. The physiochemical parameters such as pH, conductivity, total dissolved solids (TDS), dissolved oxygen (DO), chemical oxygen demand (COD) of leachate, and reference water were analyzed by using the YSI Professional Plus handheld multiparameter meter. In this study, SOD activities were decreased in the application groups compared with the Control Group at the 24th and 96th hours. CAT activities and GSH levels increased in the application groups compared with the Control Group at the 24th hour but decreased at the 96th hours. MDA levels increased in all of the application groups when compared with the Control Group for both 24 and 96 h. Different concentration of LL induces oxidative stress in P. chrysosporium, increased CAT activity and MDA levels, and decreased SOD activity and GSH levels. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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