Asarones from Acori Tatarinowii Rhizoma stimulate expression and secretion of neurotrophic factors in cultured astrocytes
| Autor: | Kelly Y.C. Lam, Huaiyou Wang, Wei-Hui Hu, Ping Yao, Karl Wah Keung Tsim, Qiyun Wu, Tina T. X. Dong |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Gene Expression Allylbenzene Derivatives Anisoles Pharmacology Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Neurotrophic factors Nerve Growth Factor Glial cell line-derived neurotrophic factor Animals Asarone Secretion Glial Cell Line-Derived Neurotrophic Factor Nerve Growth Factors Cells Cultured Brain-derived neurotrophic factor biology Chemistry Acorus Brain-Derived Neurotrophic Factor General Neuroscience Acorus tatarinowii 030104 developmental biology Nerve growth factor Astrocytes biology.protein Pka signaling Rhizome 030217 neurology & neurosurgery Drugs Chinese Herbal |
| Zdroj: | Neuroscience Letters. 707:134308 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2019.134308 |
| Popis: | Acori Tatarinowii Rhizoma (ATR, the dried rhizome of Acorus tatarinowii Schott.) is a traditional Chinese medicine widely used to treat brain diseases, e.g. depression, forgetfulness, anxiety and epilepsy. Several lines of evidence support that ATR has neuronal beneficial functions in animal models, but its action mechanism in cellular level is unknown. Here, we identified α-asarone and β-asarone could be the major active ingredients of ATR, which, when applied onto cultured rat astrocytes, significantly stimulated the expression and secretion of neurotrophic factors, i.e. nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and glial derived neurotrophic factor (GDNF), in dose-dependent manners. These results suggested that the neuronal action of ATR, triggered by asarone, might be mediated by an increase of expression of neurotrophic factors in astrocytes, which therefore could support the clinical usage of ATR. In addition, application of PKA inhibitor, H89, in cultured astrocytes partially blocked the asarone-induced neurotrophic factor expression, suggesting the involvement of PKA signaling. The results proposed that α-asarone and β-asarone from ATR could serve as potential candidates for drug development in neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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