'Universal Warming' protocol for vitrified oocytes to streamline cell exchange for transnational donation programs: a multi-center study
| Autor: | Lodovico Parmegiani, Valentina Casciani, Graciela Estela Cognigni, Marco Filicori, Ermanno Greco, Maria Giulia Minasi, R. Viñoles, L. A. Quintero, Maria Teresa Varricchio, A. Arnone |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Cryoprotectant Pregnancy Rate Oocyte Retrieval Fertilization in Vitro Andrology 03 medical and health sciences 0302 clinical medicine Human fertilization Japan Pregnancy Genetics medicine Humans Vitrification Embryo Implantation Assisted Reproduction Technologies Birth Rate Survival rate Genetics (clinical) Cryopreservation 030219 obstetrics & reproductive medicine Oocyte Donation business.industry Obstetrics and Gynecology General Medicine Oocyte cryopreservation Oocyte Embryo Transfer 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Multi center study Oocytes Female Live birth business Live Birth Developmental Biology |
| Zdroj: | J Assist Reprod Genet |
| Popis: | PURPOSE: To investigate the clinical efficacy of a “Universal Warming” protocol, based on subsequent steps with 1 M and 0.5 M concentration of extracellular cryoprotectant (ECCP), on shipped oocytes. Oocytes are vitrified using different brands of ready-to-use kits which recommend that the use of their own warming kit and combining different vitrification/warming kits may have legal consequences for assisted reproductive (AR) centers, until this practice has been validated with clinical studies. METHODS: Retrospective multi-center transnational observational study. Number of oocytes warmed 1.898. Vitrification performed with vitrification kit (Kitazato, Japan); warming carried out randomly with two different kits: Kitazato warming kit and Vit Kit®-Thaw (FujiFilm Irvine, USA). Warmed oocytes were assigned to 2 groups: KK (Kitazato/Kitazato) 939, and KI (Kitazato/Irvine) 959. Primary endpoint: survival rate. Secondary endpoints: fertilization rate; blastulation rate; implantation rate; live birth rate. RESULTS: Survival was comparable between the groups: 84.6% (795/939) in group KK vs 82.1% (787/959) in group KI. Fertilization rate was lower (P = 0.027) in group KK (75.7%—602/795) than in group KI (80.4%—633/787). Blastulation and implantation and live birth rates were all statistically comparable between the study groups: blastulation rate was 58.5% (352/602) vs 57.8% (366/633); implantation rate was 41.5% (80/193) vs 45.9% (84/183); live birth rate was 52.5% (62/118) in KK and 45.0% (54/120) in KI. CONCLUSION: The use of this “Universal Warming” protocol simplifies vitrified oocyte exchange between AR centers in different countries, and overcomes potential regulatory/commercial/availability differences affecting clinical practice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|