Cystatin 11: A New Member of the Cystatin Type 2 Family
| Autor: | Michael G. O'Rand, Gail Grossman, Perumal Sivashanmugam, Peter Petrusz, Yonglian Zhang, Rama Soundararajan, Katherine G. Hamil, Qiang Liu, Susan H. Hall, Frank S. French, Richard T. Richardson, Suresh Yenugu |
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| Rok vydání: | 2002 |
| Předmět: |
Models
Molecular DNA Complementary medicine.medical_treatment Molecular Sequence Data Microbial Sensitivity Tests Cysteine Proteinase Inhibitors Biology law.invention Exon Endocrinology law Escherichia coli medicine Animals Humans Amino Acid Sequence Northern blot Promoter Regions Genetic Protease Base Sequence Seminal Plasma Proteins Blotting Northern Epididymis Cystatins Immunohistochemistry Macaca mulatta Molecular biology Cysteine protease Recombinant Proteins medicine.anatomical_structure Biochemistry Suppression subtractive hybridization Androgens Recombinant DNA Cystatin Peptides |
| Zdroj: | Endocrinology. 143:2787-2796 |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | Cystatin (CST)11, a novel member of the CST type 2 family of cysteine protease inhibitors, was identified in Macaca mulatta epididymis by subtractive hybridization cloning. The human CST11 gene on chromosome 20p11.2 is located near three other CST genes expressed predominantly in the male reproductive tract. The CST11 gene spans three exons, a structure similar to that of other CST family 2 genes. An exon 2-deleted alternative transcript (CST112) was also identified. CST11 mRNA is expressed only in the epididymis as judged by Northern blot hybridization and is androgen regulated. The protein is most abundant in the initial segment, but is detected throughout the epididymis and on ejaculated human sperm. The calculated tertiary structure of CST11 reveals that the three regions corresponding to the protease inhibitory wedge of CST3 are similarly juxtaposed in CST11, consistent with protease inhibitor function. Intact and exon 2-deleted CST11 recombinant proteins were tested for antibacterial activity. After a 2-h incubation of Escherichia coli with 50 g/ml recombinant CST11 or CST112, bacterial colony-forming units were reduced to 30% of control, indicating that both forms have antimicrobial activity. (Endocrinology 143: 2787–2796, 2002) |
| Databáze: | OpenAIRE |
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