EXPRESSION OF IMUNOGLOBULIN, GRANZYME-B AND PERFORIN AGAINTS Ag85A AND Ag85B PROTEINS OF MYCOBACTERIUM TUBERCULOSIS IN BALB/C MICE
| Autor: | Ni Made Mertaniasih, Fihiruddin, Tri Wibawa, Wayan Tunas Artama |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
biology biology.organism_classification Molecular biology Article granzyme B and perforin Immunoglobulin G BALB/c Mycobacterium tuberculosis Granzyme B 03 medical and health sciences TB Vaccine 030104 developmental biology 0302 clinical medicine Infectious Diseases Perforin Immunoglobulin biology.protein 030211 gastroenterology & hepatology Antibody Ag85A and Ag85B proteins BCG vaccine CD8 |
| Zdroj: | African Journal of Infectious Diseases |
| ISSN: | 2505-0419 2006-0165 |
| DOI: | 10.21010/ajid.v13i2.2 |
| Popis: | Background: Ag85 is a protein that may maintain survival of M. tuberculosis in intracellular parts of host cells and is considered as a virulence factor. The expression of Ag85 protein can stimulate proliferation and differentiation of B- cells and T-cells in patients with tuberculosis. This research aimed to determine the ability of Ag85A and Ag85B proteins in activating the response of antibodies, granzyme-B and perforin in Balb/c mice. Materials and Methods: Twenty-five male Balb/c mice were assigned into five groups. Group I was treated with adjuvant, group II with Bacillus Calmette-Guerin (BCG) vaccine, group III with a combination of BCG and Ag85A, group IV with a combination of BCG and Ag85B and group V with a combination of BCG, Ag85A and Ag85B. Concentrations of immunoglobulin G, granzyme-B and perforin were examined using ELISA and the number of CD8+ T-cells and NK T-cells were checked by flow cytometry. Results: The highest concentration of immunoglobulin G was found in group V with 62.49±5.4327 ng/ml. The highest mean number of CD8+ T-cells, NK T-cells, granzyme-B and perforin was found in group IV with 4.32%, 1.03%, 35.11±1.7789 pg/ml and 6.19±0.2235 pg/ml, respectively. The results of One-Way ANOVA test showed that there were significant differences in immunoglobulin responses, with p |
| Databáze: | OpenAIRE |
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