Pollen Competition and Paternal Contribution during Artificially Controlled Pollination of Black Locust (Robinia pseudoacacia L.) without Castration
| Autor: | Qi Guo, Yingming Fan, Yun Li, Zijie Zhang, Xiuyu Li, Yuhan Sun, Ruiyang Hu, Cui Long, Sen Cao, Li Dong, Peiyao Han, Chao Han, Jiankang Li, Saleem Uddin |
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| Rok vydání: | 2021 |
| Předmět: |
biology
Pollination Offspring Selfing Zoology Forestry Outcrossing honeybee biology.organism_classification medicine.disease_cause chemistry.chemical_compound Castration chemistry hybridization breeding outcrossing rate Pollen pollen competition medicine QK900-989 without castration Mating Plant ecology Robinia pseudoacacia L artificial controlled pollination Locust |
| Zdroj: | Forests Volume 12 Issue 10 Forests, Vol 12, Iss 1416, p 1416 (2021) |
| ISSN: | 1999-4907 |
| DOI: | 10.3390/f12101416 |
| Popis: | (1) Background: Considering the serious damage caused by castration and the extremely high outcrossing rate in nature, we hypothesized that artificial controlled pollination of black locust without castration could be conducted for hybridization breeding. (2) Methods: The study conducted controlled pollination on 20 mating combinations of black locust without castration using a single or mixed male parent. Offspring of different developmental stages and the leaves of parents were collected to extract DNA and perform paternity analysis using SSR molecular markers. (3) Results: The contribution rate of each male parent differed according to developmental stage after pollination using different pollens mixed in equal proportions. There were significant correlations between the genetic similarity between each male parent and female parent and contribution rate of each male parent at three different developmental stages after pollination. (4) Conclusions: The composition of offspring pollen donors showed no bias toward selfing or outcrossing when artificially pollinated without castration. Hybrid breeding of black locust by artificially controlled pollination without castration may not be feasible, given that our manual method resulted in a large number of abortive and abnormal offspring. Introduction of honeybees in a limited space to conduct controlled pollination of black locust for hybrid breeding may be feasible. |
| Databáze: | OpenAIRE |
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