An immunoblot assay reveals that bacteriophage T4 thymidylate synthase and dihydrofolate reductase are not virion proteins
| Autor: | G. Maley, F. Maley, L. J. Wheeler, C. K. Mathews, D. H. Coombs, Xiaohong Chen |
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| Rok vydání: | 1995 |
| Předmět: |
viruses
Blotting Western Immunology Microbiology Thymidylate synthase Cell Line law.invention Bacteriophage Viral Proteins law Virology Dihydrofolate reductase Bacteriophage T4 Gene chemistry.chemical_classification Infectivity biology Immune Sera Virion Thymidylate Synthase biology.organism_classification Molecular biology Tetrahydrofolate Dehydrogenase Enzyme chemistry Biochemistry Cell culture Insect Science biology.protein Recombinant DNA Research Article |
| Zdroj: | Journal of Virology. 69:2119-2125 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.69.4.2119-2125.1995 |
| Popis: | Numerous reports describe the phage T4 enzymes thymidylate synthase and dihydrofolate reductase as structural components of the baseplate. However, Y. Wang and C. K. Mathews (J. Virol. 63:4736-4743, 1989) reported that antisera against the respective recombinant enzymes failed to neutralize phage infectivity, in contrast to previous results. Moreover, a deletion mutant lacking the genes for these two enzymes adsorbed normally to host cells. Since these findings tended to undermine the idea of the two enzymes as structural proteins, we developed a quantitative immunoblot assay to resolve the issue directly. Our results show that both enzymes are present only as minor contaminants (< 0.05 copy per phage) and as such cannot be bona fide structural proteins. |
| Databáze: | OpenAIRE |
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