A novel off–on fluorescent probe for sensitive imaging of mitochondria-specific nitroreductase activity in living tumor cells
| Autor: | Li-Juan Tang, Wei Liu, Xian-Jun Liu, Jian-Hui Jiang, Yong-Qing Kuang, Wen Chen, Bo Huang |
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| Rok vydání: | 2017 |
| Předmět: |
Indocyanine Green
Fluorophore Mitochondrion 010402 general chemistry 01 natural sciences Biochemistry HeLa chemistry.chemical_compound Nitroreductase Live cell imaging Cell Line Tumor Humans Moiety Physical and Theoretical Chemistry Fluorescent Dyes Molecular Structure biology 010405 organic chemistry Organic Chemistry Nitroreductases biology.organism_classification Fluorescence Mitochondria Molecular Imaging 0104 chemical sciences chemistry Cancer cell Biophysics HeLa Cells |
| Zdroj: | Organic & Biomolecular Chemistry. 15:4383-4389 |
| ISSN: | 1477-0539 1477-0520 |
| DOI: | 10.1039/c7ob00781g |
| Popis: | Sensitive and selective detection and imaging of nitroreductase (NTR) in cancer cells is of great importance for better understanding their biological functions. Since there are a few fluorescent probes concerning NTR activity specifically located in mitochondria, we developed a novel fluorescent benzoindocyanine probe (BICP) for mitochondrial NTR activity monitoring and imaging via extending a benzoindole moiety into a benzoindocyanine based fluorophore (BICF) with a strong intramolecular charge transfer (ICT) effect and incorporating 4-nitrobenzyl as a fluorescence-quenching and enzyme-responsive moiety. Live cell imaging of HeLa and A549 demonstrates that the developed BICP is able to realize sensitive and selective mitochondrial NTR activity probing with high-contrast "off-on" fluorescence. These findings implied the great potential of the developed probe for monitoring mitochondrial-specific NTR activities in living cells and related applications in cell biology. |
| Databáze: | OpenAIRE |
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