Dynamic regulation of chromatin organizer SATB1 via TCR-induced alternative promoter switch during T-cell development
| Autor: | Vishal Dandewad, Sanjeev Galande, Anjali Verma, Shilpi Giri, Jyoti Misra-Sen, Satyajeet P. Khare, Indumathi Patta, Girdhari Lal, Ayush Madhok, Kamalvishnu P. Gottimukkala, Vasudevan Seshadri |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
CD4-Positive T-Lymphocytes
AcademicSubjects/SCI00010 T cell T-Lymphocytes Receptors Antigen T-Cell Biology 03 medical and health sciences Mice 0302 clinical medicine Polysome Genetics medicine Animals Cell Lineage Hepatocyte Nuclear Factor 1-alpha RNA Messenger Promoter Regions Genetic Gene 030304 developmental biology 0303 health sciences Messenger RNA Thymocytes T-cell receptor Gene regulation Chromatin and Epigenetics Promoter Cell Differentiation SATB1 Matrix Attachment Region Binding Proteins Chromatin Cell biology Mice Inbred C57BL medicine.anatomical_structure 030220 oncology & carcinogenesis Protein Biosynthesis Signal Transduction |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | The chromatin organizer SATB1 is highly enriched in thymocytes and is essential for T-cell development. Although SATB1 regulates a large number of genes important for T-cell development, the mechanism(s) regulating expression of SATB1 during this process remain elusive. Using chromatin immune precipitation-seq-based occupancy profiles of H3K4me3 and H3Kme1 at Satb1 gene locus, we predicted four different alternative promoters of Satb1 in mouse thymocytes and characterized them. The expression of Satb1 transcript variants with distinct 5′ UTRs occurs in a stage-specific manner during T-cell development and is dependent on TCR signaling. The observed discrepancy between the expression levels of SATB1 mRNA and protein in developing thymocytes can be explained by the differential translatability of Satb1 transcript variants as confirmed by polysome profiling and in vitro translation assay. We show that Satb1 alternative promoters exhibit lineage-specific chromatin accessibility during T-cell development from progenitors. Furthermore, TCF1 regulates the Satb1 P2 promoter switch during CD4SP development, via direct binding to the Satb1 P2 promoter. CD4SP T cells from TCF1 KO mice exhibit downregulation of P2 transcript variant expression as well as low levels of SATB1 protein. Collectively, these results provide unequivocal evidence toward alternative promoter switch-mediated developmental stage-specific regulation of SATB1 in thymocytes. |
| Databáze: | OpenAIRE |
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