Clinical sensitivity of HPV assays for the detection of high grade cervical disease in cervical samples treated with glacial acetic acid
| Autor: | Catherine Moore, Cat Graham, Logan Grieve, Kate Cuschieri, G. L. Reid, Edward Duvall, Heather Cubie, Eileen Docherty, Ellen Braby |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Pathology Dyskaryosis 030106 microbiology Uterine Cervical Neoplasms Cervical disease Cervix Uteri Hpv detection Gastroenterology Sensitivity and Specificity Specimen Handling 03 medical and health sciences 0302 clinical medicine Virology Internal medicine parasitic diseases medicine Humans Prospective Studies Papillomaviridae Acetic Acid Hpv types business.industry Hybrid capture Papillomavirus Infections medicine.disease female genital diseases and pregnancy complications Hpv testing Infectious Diseases Molecular Diagnostic Techniques High risk hpv 030220 oncology & carcinogenesis Liquid-based cytology Female Indicators and Reagents business |
| Zdroj: | Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 79 |
| ISSN: | 1873-5967 |
| Popis: | Background Lysis of bloody liquid based cytology (LBC) specimens with glacial acetic acid (GAA) is performed to aid cytological interpretation. However, the influence of GAA treatment on HPV detection is not fully understood and in studies designed to assess this, few cases of high-grade disease have been included. Objectives To assess the sensitivity of HPV molecular tests for the detection of high grade cervical disease in GAA treated samples Study design A total of 207 specimens associated with high grade dyskaryosis and treated with GAA were collated prospectively. Overall 140 specimens had underlying CIN2+, including 88 CIN3. All specimens were tested with the Abbott RealTime High Risk HPV test ( rt HPV) and the Qiagen Hybrid Capture 2High Risk HPV DNA test (HC2). Specimens associated with a CIN2+ that were negative by either assay were genotyped. Results The sensitivity of rt HPV for CIN2+ and CIN3+ was 92.8% (87.2, 96.5) and 94.3% (87.2, 98.1) respectively. Sensitivity of the HC2 for CIN2+ and CIN3+ was 97.2% (92.8, 99.2) and 96.6% (90.3, 99.2) respectively. The sensitivity of both assays in GAA treated specimens was thus consistent with the level required for clinical application. HPV negative, CIN2+ specimens were generally attributable to HPV types outside the explicit analytical range of the assays. Conclusions The data indicate that GAA treatment has little impact on the detection of CIN2+ by HPV testing in LBC specimens. |
| Databáze: | OpenAIRE |
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