The expression and localization of Toll-like receptors 2, 4, 5 and 9 in the epididymis and vas deferens of a adult tom cats
| Autor: | Emel Alan, Narin Liman, Nusret Apaydin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Blotting Western Biology Andrology 03 medical and health sciences Vas Deferens 0302 clinical medicine Food Animals medicine Animals Small Animals Receptor Epididymis Toll-like receptor 030219 obstetrics & reproductive medicine CATS Equine Toll-Like Receptors 0402 animal and dairy science Vas deferens 04 agricultural and veterinary sciences Immunohistochemistry 040201 dairy & animal science Toll-Like Receptor 2 Toll-Like Receptor 4 Blot Toll-Like Receptor 5 TLR2 medicine.anatomical_structure Toll-Like Receptor 9 Cats Animal Science and Zoology |
| Popis: | Toll-like receptors (TLRs) are important molecules, which provide protection against infections of the reproductive tract. This study demonstrates for the first time the expression and localization patterns of TLRs in the caput, corpus and cauda segments of the epididymal duct (ED) and the vas deferens (VD) of adult domestic cats using immunohistochemistry and western blotting. While immunoblot analyses revealed relatively similar protein levels for TLRs 2, 4, 5, and 9 in three segments of the ED, the protein levels of TLR2 and TLR4 in the VD were found to be significantly higher than those measured in the ED segments (P < 0.05). On the other hand, immunostaining showed that TLRs exhibited regional- and cell-specific localization patterns. TLR2 and TLR5 were immunolocalized to the nucleus and cytoplasm of the principal cells in all ducts. TLR4 was restricted to the stereocilia, and TLR9 was located in the cytoplasm ofthe principal cells. Narrow cells displayed positive immunoreactions for TLR4 and TLR5. The basal cells of the different ED segments were positive for all four TLRs. TLR2, TLR5 and TLR9 were detected in the cytoplasmic droplets of the spermatozoa. TLR4 and TLR9 were detected along the entire length of the sperm tail, whilst TLR2 and TLR5 were absent in the midpiece. TLR2 and TLR5 were also detected in the equatorial segment of the sperm head. These results suggest that TLR2, TLR4, TLR5,and TLR9 are important not only for the protection of the ED, VD and spermatozoa but also for the maturation and storage of spermatozoa in the ED and VD, respectively. |
| Databáze: | OpenAIRE |
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