Culture of Borrelia persica and its Flagellar Antigen in vitro
| Autor: | Seyed Hossein Hosseini, Mohammad Soleimani, Fatemeh Pourfallah, Zahra Zamani, Farzad Oreiz, Mohammad Arjmand, Reza Saghiri, Saifeddin Javadian, Mehdi Assmar |
|---|---|
| Přispěvatelé: | Department of Biochemistry, Réseau International des Instituts Pasteur - Institut Pasteur d'Iran, Department of Parasitology, Institut Pasteur d'Iran, Réseau International des Instituts Pasteur - Institut Pasteur d'Iran - Réseau International des Instituts Pasteur - Institut Pasteur d'Iran, Department of Immunology, Razi institute of Serology, Institute Pasteur of Iran, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) |
| Rok vydání: | 2014 |
| Předmět: |
relapsing fever
Cross Reactions medicine.disease_cause MESH: Flagella 01 natural sciences Cross-reactivity Microbiology MESH: Cross Reactions 03 medical and health sciences Antigen Borrelia Antigenic variation medicine MESH: Borrelia [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology 030304 developmental biology Antigens Bacterial 0303 health sciences Borrelia persica biology Chemistry 010401 analytical chemistry Periplasmic space biology.organism_classification medicine.disease 3. Good health 0104 chemical sciences [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology indirect haemmaglutination kit Flagella Spirochaete Bacterial outer membrane Agronomy and Crop Science MESH: Antigens Bacterial |
| Zdroj: | Pakistan Journal of Biological Sciences Pakistan Journal of Biological Sciences, Asian Network for Scientific Information (ANSINET), 2014, 17 (2), pp.190-7. Pakistan Journal of Biological Sciences, Asian Network for Scientific Information (ANSINET), 2014, 17 (2), pp.190-7. ⟨10.3923/pjbs.2014.190.197⟩ |
| ISSN: | 1028-8880 1812-5735 |
| Popis: | International audience; Borrelia persica is a strain seen only in the Middle East and responsible for relapsing fever. These spirochetes are notable for multiphasic antigenic variation of polymorphic outer membrane lipoproteins, a phenomenon responsible for immune evasion. Diagnosis of the disease is a problem and requires a fixed antigen like the flagellar antigen. In vitro culture of B. persica was carried out for the first time and flagellar antigen was purified from culture. 10% SDS was added to the mixture to dissolve the cell wall and then the solution was sheared in an Omni mixer. Electron microscopy confirmed the purity of a 42 KDa periplasmic antigen as revealed by SDS-PAGE. Indirect haemagglutination kits were designed using the pure flagella and tested for cross reactivity with another relapsing fever spirochaete Borrelia microtii positive serum. The kit showed 98% sensitivity and 95% specificity. |
| Databáze: | OpenAIRE |
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