Coordinated regulation of genes for secretion in tobacco at late developmental stages: Association with resistance against oomycetes
| Autor: | Chimène Moreilhon, Gilles Arbiol, Manal A. Dayem, Catherine Weiss, Marie-Pierre Rivière, Eric Galiana, Karine Hugot, Joseph Cozzitorto, Pascal Barbry |
|---|---|
| Přispěvatelé: | Interactions plantes-microorganismes et santé végétale (IPMSV), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Kervella, Jacqueline, ProdInra, Migration, Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS) |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
0106 biological sciences
Physiology Nicotiana tabacum Plant Science Age-related resistance Biology 01 natural sciences [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics 03 medical and health sciences [SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics Gene expression Botany Genetics Extracellular Gene ComputingMilieux_MISCELLANEOUS 030304 developmental biology [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism 2. Zero hunger Regulation of gene expression Oomycete 0303 health sciences fungi NORTHERN BLOT [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism biology.organism_classification Cell biology Systemic acquired resistance 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2004, 134, pp.858-870 HAL |
| ISSN: | 0032-0889 1532-2548 |
| Popis: | Besides the systemic acquired resistance (SAR) induced in response to microbial stimulation, host plants may also acquire resistance to pathogens in response to endogenous stimuli associated with their own development. In tobacco (Nicotiana tabacum), the vegetative-to-flowering transition comes along with a susceptibility-to-resistance transition to the causal agent of black shank disease, the oomycete Phytophthora parasitica. This resistance affects infection effectiveness and hyphal expansion and is associated with extracellular accumulation of a cytotoxic activity that provokes in vitro cell death of P. parasitica zoospores. As a strategy to determine the extracellular events important for restriction of pathogen growth, we screened the tobacco genome for genes encoding secreted or membrane-bound proteins expressed in leaves of flowering plants. Using a signal sequence trap approach in yeast (Saccharomyces cerevisiae), 298 clones were selected that appear to encode for apoplastic, cell wall, or membrane-bound proteins involved in stress response, in plant defense, or in cell wall modifications. Microarray and northern-blot analyses revealed that, at late developmental stages, leaves were characterized by the coordinate up-regulation of genes involved in SAR and in peroxidative cross-linking of structural proteins to cell wall. This suggests the potential involvement of these genes in extracellular events that govern the expression of developmental resistance. The analysis of the influence of salicylic acid on mRNA accumulation also indicates a more complex network for regulation of gene expression at a later stage of tobacco development than during SAR. Further characterization of these genes will permit the formulation of hypotheses to explain resistance and to establish the connection with development. |
| Databáze: | OpenAIRE |
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