Mutational analysis of the 5′ non-coding region of human immunodeficiency virus type 1: effects of secondary structure on translation
Autor: | A Darveau, N T Parkin, W Haseltine, Éric A. Cohen, Nahum Sonenberg, C Rosen |
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Rok vydání: | 1988 |
Předmět: |
Chloramphenicol O-Acetyltransferase
Untranslated region Microinjections Transcription Genetic Five prime untranslated region Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology Xenopus laevis medicine Protein biosynthesis Animals Humans Coding region RNA Messenger Molecular Biology Protein secondary structure Genetics Mutation Messenger RNA General Immunology and Microbiology General Neuroscience Translation (biology) Protein Biosynthesis HIV-1 Oocytes Nucleic Acid Conformation RNA Viral Female Plasmids Research Article |
Zdroj: | The EMBO Journal. 7:2831-2837 |
ISSN: | 0261-4189 |
Popis: | The first 111 nt from the 5' end of human immunodeficiency virus type 1 (HIV-1) mRNAs are shown to have a strong inhibitory effect on the translation of mRNA in in vitro translation extracts as well as in Xenopus oocytes. Mutations in the sequence of the 5' untranslated region (UTR) designed to disrupt predicted secondary structure of this region relieve the inhibition. Inhibition is restored by mutations that reconstruct the predicted secondary structure. The accessibility of the 5'-terminal cap structure was also found to be increased by some of these mutations. We conclude that secondary structure in the 5' UTR of HIV-1 mRNAs and resultant inaccessibility of the cap structure is responsible for the inhibition of translation. The implications of these findings for the understanding of the life cycle of HIV-1 are discussed. |
Databáze: | OpenAIRE |
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