Menstrual flow as a non-invasive source of endometrial organoids
| Autor: | Ashley Moffett, Graham J. Burton, Margherita Y. Turco, Kay Elder, Carolyn J.P. Jones, Tereza Cindrova-Davies, Xiaohui Zhao |
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| Přispěvatelé: | Cindrova-Davies, Tereza [0000-0002-9212-0514], Zhao, Xiaohui [0000-0001-9922-2815], Jones, Carolyn JP [0000-0002-0026-9494], Moffett, Ashley [0000-0002-8388-9073], Burton, Graham J [0000-0001-8677-4143], Turco, Margherita Y [0000-0002-3380-7375], Apollo - University of Cambridge Repository, Jones, Carolyn J. P. [0000-0002-0026-9494], Burton, Graham J. [0000-0001-8677-4143], Turco, Margherita Y. [0000-0002-3380-7375], Jones, Carolyn J P [0000-0002-0026-9494] |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Receptor expression Endometriosis Medicine (miscellaneous) Pilot Projects 692/1807/2782 Endometrium 13 14 0302 clinical medicine Recurrent miscarriage Biology (General) reproductive and urinary physiology Cells Cultured medicine.diagnostic_test 631/443/494/2732/1577 Biological techniques Menstruation Organoids medicine.anatomical_structure 030220 oncology & carcinogenesis Female General Agricultural and Biological Sciences Reproductive signs and symptoms Adult Cell biology QH301-705.5 13/106 Fertilization in Vitro General Biochemistry Genetics and Molecular Biology Article Andrology 14/1 03 medical and health sciences Biopsy medicine Organoid Humans 101/1 In vitro fertilisation 631/1647 business.industry 82 medicine.disease 030104 developmental biology Infertility 13/51 14/63 631/80 business Hormone |
| Zdroj: | Communications Biology Communications Biology, Vol 4, Iss 1, Pp 1-8 (2021) Cindrova-Davies, T, Zhao, X, Elder, K, Jones, C J P, Moffett, A, Burton, G J & Turco, M Y 2021, ' Menstrual flow as a non-invasive source of endometrial organoids ', Communications Biology . https://doi.org/10.1038/s42003-021-02194-y |
| Popis: | Funder: Centre for Trophoblast Research Assessment of the endometrium often necessitates a biopsy, which currently involves an invasive, transcervical procedure. Here, we present an alternative technique based on deriving organoids from menstrual flow. We demonstrate that organoids can be derived from gland fragments recovered from menstrual flow. To confirm they faithfully reflect the in vivo state we compared organoids derived from paired scratch biopsies and ensuing menstrual flow from patients undergoing in vitro fertilisation (IVF). We demonstrate that the two sets of organoids share the same transcriptome signature, derivation efficiency and proliferation rate. Furthermore, they respond similarly to sex steroids and early-pregnancy hormones, with changes in morphology, receptor expression, and production of ‘uterine milk’ proteins that mimic those during the late-secretory phase and early pregnancy. This technique has wide-ranging impact for non-invasive investigation and personalised approaches to treatment of common gynaecological conditions, such as endometriosis, and reproductive disorders, including failed implantation after IVF and recurrent miscarriage. |
| Databáze: | OpenAIRE |
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