The Cassava Genome: Current Progress, Future Directions
| Autor: | Steve Rounsley, Brian Desany, Timothy T. Harkins, Daniel S. Rokhsar, Mohammed Mohiuddin, Chinnappa D. Kodira, Joe Tohme, Simon Prochnik, Pablo D. Rabinowicz, Claude M. Fauquet, Pradeep Reddy Marri, Fausto J. Rodriguez |
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| Rok vydání: | 2012 |
| Předmět: |
Whole genome sequencing
Genetics Cassava education.field_of_study Expressed sequence tag Shotgun sequencing Population food and beverages Genomics Crop improvement Plant Science Biology Genome Article DNA sequencing Linkage mapping Genotyping by sequencing 454-sequencing Polymorphism education Genome size |
| Zdroj: | Tropical Plant Biology |
| ISSN: | 1935-9764 1935-9756 |
| Popis: | The starchy swollen roots of cassava provide an essential food source for nearly a billion people, as well as possibilities for bioenergy, yet improvements to nutritional content and resistance to threatening diseases are currently impeded. A 454-based whole genome shotgun sequence has been assembled, which covers 69% of the predicted genome size and 96% of protein-coding gene space, with genome finishing underway. The predicted 30,666 genes and 3,485 alternate splice forms are supported by 1.4 M expressed sequence tags (ESTs). Maps based on simple sequence repeat (SSR)-, and EST-derived single nucleotide polymorphisms (SNPs) already exist. Thanks to the genome sequence, a high-density linkage map is currently being developed from a cross between two diverse cassava cultivars: one susceptible to cassava brown streak disease; the other resistant. An efficient genotyping-by-sequencing (GBS) approach is being developed to catalog SNPs both within the mapping population and among diverse African farmer-preferred varieties of cassava. These resources will accelerate marker-assisted breeding programs, allowing improvements in disease-resistance and nutrition, and will help us understand the genetic basis for disease resistance. |
| Databáze: | OpenAIRE |
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