Diffuse mesenterial sclerosis: a characteristic feature of chronic small-bowel allograft rejection
| Autor: | Alexander Klaus, Heinz Pernthaler, G. Klima, Raimund Margreiter, Felix Offner |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Graft Rejection
Male Vasculitis Pathology medicine.medical_specialty Time Factors medicine.medical_treatment H&E stain Sclerosing mesenteritis Pathology and Forensic Medicine Submucosa Cyclosporin a Intestine Small Animals Transplantation Homologous Medicine Mesentery Intestinal Mucosa Mesenteries Molecular Biology Sclerosis Crypt Epithelium business.industry Body Weight Immunosuppression Cell Biology General Medicine medicine.disease Rats Rats Inbred ACI Transplantation Disease Models Animal medicine.anatomical_structure Rats Inbred Lew Chronic Disease Cyclosporine Leukocytes Mononuclear Blood Vessels business Immunosuppressive Agents |
| Zdroj: | Virchows Archiv. 442:48-55 |
| ISSN: | 1432-2307 0945-6317 |
| DOI: | 10.1007/s00428-002-0715-9 |
| Popis: | Chronic rejection is the major cause of late intestinal allograft dysfunction. The aim of this study was to analyze in detail the histopathological features of chronic rejection in the ACI-to-Lewis rat model of intestinal transplantation. Chronic rejection was achieved in orthotopic small-bowel allografts (ACI-Lewis) by limited immunosuppression with cyclosporin A (CyA). Isogeneic transplants (ACI-ACI) as well as native bowels (ACI) with and without immunosuppression served as controls. Bowels were removed together with the mesenteries 90 days postoperatively and analyzed using sections stained with hematoxylin and eosin as well as Masson's trichrome. The slides were coded, randomized and analyzed by grading of histological abnormalities. The most striking alterations of the allografts were noticed in the mesenteries exhibiting an extensive infiltration by mononuclear cells accompanied by a progressive diffuse fibrosis with shrinking of the mesenteries. These changes were most pronounced in the perivascular areas of the mesenteric arteriae and venae rectae. Three of five allografts showed vasculitis with myointimal proliferation of the arteriae rectae. Focally, there was spill-over of the inflammatory cells onto the intestinal muscularis propria. The mucosa of the allografts showed mild blunting, lymphocytic infiltration of the crypt epithelium and increased crypt cell apoptoses. The submucosa was unaffected, and there were no detectable abnormalities of the enteric ganglion cells. The present data support the view that chronic rejection of intestinal allografts is characterized by a diffuse sclerosing mesenteritis which may significantly contribute to late graft dysfunction. The present model may be useful to study the pathomechanisms of this inflammatory fibrosing process. |
| Databáze: | OpenAIRE |
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