82-FIP, a novel FMRP (Fragile X Mental Retardation Protein) interacting protein, shows a cell cycle-dependent intracellular localization
| Autor: | Bardoni B, Castets M, Huot ME, Schenck A, Adinolfi S, Corbin F, Pastore A, Khandjian EW, Mandel JL, RI Schenck Annette/E-4514-2012 |
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| Přispěvatelé: | Bardoni, B, Castets, M, Huot, Me, Schenck, A, Adinolfi, S, Corbin, F, Pastore, A, Khandjian, Ew, Mandel, Jl, RI Schenck, Annette/E-4514-2012 |
| Rok vydání: | 2003 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Messenger-RNA knock-out mice identification gene nuclear association translation mouse model MRNP mouse model MRNP Cell translation Nerve Tissue Proteins RNA-binding protein Biology Fragile X Mental Retardation Protein Messenger-RNA RNA interference Polysome Genetics medicine Humans gene Molecular Biology Genetics (clinical) Cell Cycle association Nuclear Proteins RNA-Binding Proteins RNA General Medicine Cell cycle Molecular biology nervous system diseases Cell biology nuclear Cell nucleus medicine.anatomical_structure Cytoplasm Polyribosomes knock-out mice identification Carrier Proteins Protein Binding |
| Zdroj: | Human Molecular Genetics. 12:1689-1698 |
| ISSN: | 1460-2083 |
| DOI: | 10.1093/hmg/ddg181 |
| Popis: | FMRP is an RNA binding protein whose absence produces pathological manifestations of the fragile-X syndrome. FMRP is a component of mRNP complexes found in association with actively translating polyribosomes, RNA complexes trafficking in neurites, RNA granules in cytoplasm and, in Drosophila, with the RNAi machinery. We report here the identification and characterization of a novel FMRP-interacting protein associated to polyribosomes as a component of mRNP complexes containing FMRP. We named this protein 82-FIP (82-kD FMRP Interacting Protein). FMRP interacts with 82-FIP through a novel interaction motif located in its N-terminal region. The distribution of 82-FIP in different areas of the brain is very similar to that of FMRP. However, unlike FMRP, 82-FIP is found in both nucleus and cytoplasm in some neurons, while it appears only cytoplasmic in others. Subcellular distribution of 82-FIP is cell cycle-dependent in cultured cells, suggesting that the composition of some FMRP-containing RNP complexes may be cell cycle-modulated. |
| Databáze: | OpenAIRE |
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