Evaluation of the diagnostic performances of two commercially available assays for the detection of enteric adenovirus antigens
| Autor: | Chiara Filizzolo, Donatella Ferraro, Francesca Di Bernardo, Floriana Bonura, Antonella Collura, Vito Martella, Simona De Grazia, Giovanni M. Giammanco, Chiara Mascarella, Celestino Bonura |
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| Přispěvatelé: | Bonura F., Mascarella C., Filizzolo C., Bonura C., Ferraro D., Di Bernardo F., Collura A., Martella V., Giammanco G.M., De Grazia S. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
medicine.medical_specialty Settore MED/07 - Microbiologia E Microbiologia Clinica Adolescent Adenoviridae Infections Concordance Sensitivity and Specificity Gastroenterology Adenoviridae Feces Antigen Chemiluminescent immunoassay Internal medicine Fecal antigens detection medicine Humans Child Antigens Viral Immuno chromatography Acute gastroenteriti business.industry Significant difference Infant Newborn Enteric adenoviruse Infant General Medicine Acute gastroenteritis Gastroenteritis Hospitalization Infectious Diseases Italy Child Preschool Luminescent Measurements Reagent Kits Diagnostic Diagnostic performance business Viral load |
| Popis: | The performance of 2 antigenic commercial assays for enteric adenovirus (AdV) infection, bioNexia Rota-Adeno ImmunoChromatographic Tests (ICT) and LIAISON® Adenovirus ChemiLuminescence Immuno Assays (CLIA), was evaluated on 321 stools from children hospitalized for acute gastroenteritis in Palermo, Italy, using a Real time-PCR (Rt-PCR) as reference method. The CLIA showed higher sensitivity (77% vs 60%), accuracy (94.4 vs 90.9) and concordance (k: 0.81 vs 0.67) with respect to ICT, despite equivalent specificity (98.8%). Using the Ct values of the Rt-PCR as a proxy of the fecal viral load, similar Ct values (mean 9.32 vs 9.89) were observed among the true positive samples, whilst a significant difference (P < 0.05) was observed in false negative samples of CLIA (mean Ct 25.68) and ICT (mean Ct 19.87). Cross-reactivity with other enteric viruses was not observed. These results indicate that both the assays tested are suitable for diagnosis of AdV gastroenteritis. |
| Databáze: | OpenAIRE |
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