Prophylactic administration of chicken cathelicidin-2 boosts zebrafish embryonic innate immunity
| Autor: | Schneider, Viktoria A F, van Dijk, Albert, van der Sar, Astrid M, Kraaij, Marina D, Veldhuizen, Edwin J A, Haagsman, Henk P, LS Moleculaire Afweer, Dep Infectieziekten Immunologie, Infection & Immunity, dI&I I&I-3 |
|---|---|
| Přispěvatelé: | Medical Microbiology and Infection Prevention, AII - Infectious diseases, LS Moleculaire Afweer, Dep Infectieziekten Immunologie, Infection & Immunity, dI&I I&I-3 |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian animal structures Salmonella enteritidis medicine.medical_treatment Immunology Drug Evaluation Preclinical Zebrafish embryos Cathelicidin Microbiology Fish Diseases 03 medical and health sciences 0302 clinical medicine Adjuvants Immunologic medicine Animals Zebrafish Cell Proliferation Phagocytes Salmonella Infections Animal Immune stimulation Innate immune system biology Cell growth Lethal dose Embryo Chicken cathelicidin-2 biology.organism_classification Embryonic stem cell Virology Immunity Innate 030104 developmental biology embryonic structures Host defense peptide 030217 neurology & neurosurgery Antimicrobial Cationic Peptides Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology, 60, 108-114. Elsevier Limited Developmental and Comparative Immunology, 60, 108. Elsevier Limited Schneider, V A F, van Dijk, A, van der Sar, A M, Kraaij, M D, Veldhuizen, E J A & Haagsman, H P 2016, ' Prophylactic administration of chicken cathelicidin-2 boosts zebrafish embryonic innate immunity ', Developmental & Comparative Immunology, vol. 60, pp. 108-114 . https://doi.org/10.1016/j.dci.2016.02.023 |
| ISSN: | 0145-305X |
| DOI: | 10.1016/j.dci.2016.02.023 |
| Popis: | Chicken cathelicidin-2 (CATH-2) is a host defense peptide that exhibits immunomodulatory and antibacterial properties. Here we examined effects of CATH-2 in zebrafish embryos in the absence and presence of infection. Yolk-injection of 0.2–1.5 h post-fertilized (hpf) zebrafish embryos with 2.6 ng/kg CATH-2 increased proliferation of phagocytic cells at 48 hpf by 30%. A lethal infection model was developed to test the prophylactic protective effect of CATH-2 peptide. Embryos (0.2–1.5 hpf) were injected with 2.6 ng/kg CATH-2, challenged with a lethal dose of fluorescently labeled Salmonella enteritidis pGMDs3 at 28 hpf and monitored for survival. Prophylactic treatment with CATH-2 was found to delay infection starting at 22 h post-infection (hpi). At 18–20 hpi, significantly lower (2-fold) fluorescence intensity and decreased bacterial loads were detected in peptide-treated embryos. Thus prophylactic administration of low CATH-2 concentrations confer partial protection in zebrafish embryos by boosting the innate immune system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect