Phosphoglycerate mutase knock-out mutant Saccharomyces cerevisiae: Physiological investigation and transcriptome analysis

Autor: Intawat Nookaew, Jens Nielsen, Verena Siewers, Gionata Scalcinati, Marta Papini
Přispěvatelé: Systems Biology, Chalmers University of Technology [Göteborg]
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Zdroj: Biotechnology Journal
Biotechnology Journal, Wiley-VCH Verlag, 2010, 6 (3), pp.1016. ⟨10.1002/biot.201000199⟩
ISSN: 1860-6768
1860-7314
DOI: 10.1002/biot.201000199
Popis: International audience; The yeast Saccharomyces cerevisiae is able to adapt its metabolism to grow on different carbon sources and shift to non-fermentative growth on C2 or C3 carbon sources (ethanol, acetate or glycerol) requires activation of gluconeogenesis. Here we studied the response to deletion of the glycolytic and gluconeogenic gene GPM1, encoding for phosphoglycerate mutase. It was previously shown that a S. cerevisiae strain with non-functional copies of GPM1 only grow when glycerol and ethanol are both present as carbon source, however, if glucose is added, growth is strongly inhibited. It was suggested that glycerol is needed to feed gluconeogenesis whereas ethanol is used for respiration. Here we studied the physiological response of deletion of the GPM1 knock-out mutant through fermentation and transcriptome analysis. We compared the physiological results with those obtained by simulations using a genome-scale metabolic model, showing that glycerol is needed for growth only in small amounts. Our findings strongly indicate a severely impaired growth ability of the knock-out mutant, which presents increased transcript levels of genes involved in the pentose phosphate pathway and in the glyoxylate shunt. These results indicate that the mutant is attempting to compensate for the energy imbalance caused by the deletion of the glycolytic/gluconeogenic gene.
Databáze: OpenAIRE
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