Circulating clonotypic B cells in multiple myeloma and monoclonal gammopathy of undetermined significance
| Autor: | Julia Almeida, M. Jara-Acevedo, Alberto Orfao, María Eugenia Sarasquete, Martin Perez-Andres, Marcos González, Paloma Bárcena, Bruno Paiva, Leandro S. Thiago, Jesús F. San Miguel, Ana Balanzategui, Ramón García-Sanz, Maria Luz Sanchez |
|---|---|
| Přispěvatelé: | Ministerio de Sanidad y Consumo (España), Asociación Española Contra el Cáncer, Ministério da Educação (Brasil), Governo Brasil, European Commission, Red Temática de Investigación Cooperativa en Cáncer (España), Instituto de Salud Carlos III, Junta de Castilla y León, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil) |
| Rok vydání: | 2013 |
| Předmět: |
Plasma Cells
B-Lymphocyte Subsets Receptors Antigen B-Cell Monoclonal Gammopathy of Undetermined Significance Immunophenotyping Igh locus Oligonucleotide polymerase medicine Humans Lymphocyte Count Multiple myeloma B-Lymphocytes Chemistry Articles Hematology medicine.disease Molecular biology Peripheral blood Clone Cells medicine.anatomical_structure Immunology Monoclonal Bone marrow Stem cell Multiple Myeloma Monoclonal gammopathy of undetermined significance |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1592-8721 0390-6078 |
| DOI: | 10.3324/haematol.2013.092817 |
| Popis: | This is an open-access paper. The B-cell compartment in which multiple myeloma stem cells reside remains unclear. We investigated the potential presence of mature, surface-membrane immunoglobulin-positive B lymphocytes clonally related to the tumor bone marrow plasma cells among different subsets of peripheral blood B cells from ten patients (7 with multiple myeloma and 3 with monoclonal gammopathies of undetermined significance). The presence of clonotypic immunoglobulin heavy chain gene rearrangements was determined in multiple highly-purified fractions of peripheral blood B-lymphocytes including surface-membrane IgM+ CD27- naïve B-lymphocytes, plus surface-membrane IgG+, IgA+ and IgM+ memory CD27+ B cells, and normal circulating plasma cells, in addition to (mono)clonal plasma cells, by a highly-specific and sensitive allele-specific oligonucleotide polymerase chain reaction directed to the CDR3 sequence of the rearranged IGH gene of tumor plasma cells from individual patients. Our results showed systematic absence of clonotypic rearrangements in all the different B-cell subsets analyzed, including M-compo-nent isotype-matched memory B-lymphocytes, at frequencies This work was supported by grants from European Union FP6 STREP MSCNet (N. E06005FF), Cooperative Research Thematic Network on Cancer (RTICs; RTICC RD06/0020/0035-FEDER, RD06/0020/0006, RD12/0036/0048, RD12/0036/0069 and G03/136), Instituto de Salud Carlos III/Subdirección General de Investigación Sanitaria Ministerio de Sanidad y Consumo (FIS: PI060339; 02/0905; 01/0089/01-02;PS09/01897, and PI06/0824-FEDER), Asociacion Española Contra el Cancer AECC (GCB120981SAN) and Gerencia Regional de Salud de Castilla y León; Ayuda de Excelencia de Castilla y León, Consejería de Educación (EDU/894/2009, GR37) Junta de Castilla y León, Valladolid, Spain. LST received a CAPES/Ministério da Educação scholarship from the Brazilian Government. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|