High-Throughput Lipolysis in 96-Well Plates for Rapid Screening of Lipid-Based Drug Delivery Systems
| Autor: | Thomas Rades, Anette Müllertz, Huiling Mu, Philip Jonas Sassene, Mette Dalskov Mosgaard |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Cinnarizine
Time Factors Lipolysis Pharmaceutical Science 02 engineering and technology Pharmacology 030226 pharmacology & pharmacy Models Biological In vitro model 03 medical and health sciences 0302 clinical medicine Drug Delivery Systems medicine Danazol Chemistry Aqueous two-phase system 021001 nanoscience & nanotechnology Lipid Metabolism Lipids High-Throughput Screening Assays Solubilization Drug delivery 0210 nano-technology medicine.drug |
| Zdroj: | Journal of pharmaceutical sciences. 106(4) |
| ISSN: | 1520-6017 |
| Popis: | The high-throughput in vitro intestinal lipolysis model (HTP) applicable for rapid and low-scale screening of lipid-based drug delivery systems (LbDDSs) was optimized and adjusted as to be conducted in 96-well plates (HTP-96). Three different LbDDSs (I-III) loaded with danazol or cinnarizine were used as model systems. The distributions of cinnarizine and danazol in the aqueous and precipitated digestion phases generated during lipolysis in HTP-96 were compared with previously published data obtained from HTP. The final HTP-96 setup resulted in the same rank order as the original HTP model with regard to solubilization in the aqueous phase during digestion: LbDDS III > LbDDS II > LbDDS I for danazol and LbDDS III ≈ LbDDS II ≈ LbDDS I for cinnarizine. HTP-96 is a useful model for fast performance assessment of LbDDS in a small scale. |
| Databáze: | OpenAIRE |
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