Novel nonribosomal peptide synthetase (NRPS) genes sequenced from intertidal mudflat bacteria
| Autor: | Sandrine Didelot, Fatoumata Tambadou, Ibtissem Doghri, Cyrille Barthélémy, Géraldine L. Klein, Valérie Sopena, Sophie Sablé, Valérie Thiéry, Romain Chevrot, Isabelle Lanneluc |
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| Přispěvatelé: | LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
DNA
Bacterial Geologic Sediments [SDV.BIO]Life Sciences [q-bio]/Biotechnology Firmicutes Molecular Sequence Data Bacillus Microbial Sensitivity Tests Gram-Positive Bacteria DNA Ribosomal Microbiology Enterococcus faecalis 03 medical and health sciences Anti-Infective Agents Nonribosomal peptide RNA Ribosomal 16S Antibiosis Proteobacteria Genetics 14. Life underwater Peptide Synthases Molecular Biology Gene ComputingMilieux_MISCELLANEOUS 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology 030306 microbiology Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology chemistry France [SDE.BE]Environmental Sciences/Biodiversity and Ecology Bacteria |
| Zdroj: | FEMS Microbiology Letters FEMS Microbiology Letters, Wiley-Blackwell, 2014, pp.n/a-n/a. ⟨10.1111/1574-6968.12532⟩ |
| ISSN: | 0378-1097 1574-6968 |
| DOI: | 10.1111/1574-6968.12532⟩ |
| Popis: | Nonribosomal peptide synthetases (NRPS) are actively sought out, due to pharmacologically important activities of their metabolites. In marine environment, the most prevalent nonribosomal peptide antibiotic producers are sponges inhabiting microorganisms. Conversely, strains from marine sediments and more especially from intertidal mudflats have not been extensively screened for the presence of new NRPS. In this study, for the first time, a collection of one hundred intertidal mudflat bacterial isolates (Marennes-Oleron Bay, France) was assessed for (1) the presence of NRPS genes by degenerated PCR targeting conserved adenylation domains and (2) for their production of antimicrobial molecules. (1) Bacteria with adenylation domains (14 strains) were identified by 16S rRNA gene sequence analysis and grouped into Firmicutes (one strain) and Proteobacteria (13 strains). In silico analysis of the NRPS amino acid sequences ( n = 7) showed 41–58% ID with sequences found in the NCBI database. Three new putative adenylation domain signatures were found. (2) The culture supernatant of one of these strains, identified as a Bacillus, was shown to strongly inhibit the growth of Staphylococcus aureus, S. epidermidis, and Enterococcus faecalis . This study portends that the intertidal mudflat niche could be of interest for the discovery of new NRPS genes and antimicrobial producing strains. |
| Databáze: | OpenAIRE |
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