Generation of donor natural killer cells from CD34+ progenitor cells and subsequent infusion after HLA-mismatched allogeneic hematopoietic cell transplantation: a feasibility study
| Autor: | S R Yoon, Yung Sang Lee, Dae Yeon Kim, K H Ahn, Kyu-Bo Lee, I Choi, Y. Kang, J W Chung, Ju-H Lee, Seong-Gil Ryu, Mijin Jeon, Miee Seol, S H Yang, Je-H Lee |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Cell Culture Techniques CD34 Graft vs Host Disease Antigens CD34 Natural killer cell Young Adult HLA Antigens Humans Transplantation Homologous Medicine Progenitor cell Transplantation Acute leukemia Leukemia business.industry Hematopoietic Stem Cell Transplantation Hematology Leukapheresis Middle Aged Hematopoietic Stem Cells Tissue Donors Killer Cells Natural Treatment Outcome medicine.anatomical_structure Cell culture Lymphocyte Transfusion Myelodysplastic Syndromes Immunology Feasibility Studies Female Stem cell business |
| Zdroj: | Bone Marrow Transplantation. 45:1038-1046 |
| ISSN: | 1476-5365 0268-3369 |
| DOI: | 10.1038/bmt.2009.304 |
| Popis: | Post transplant infusion of donor-type natural killer (NK) cells has been shown to have an anti-leukemia-enhancing effect without evoking GVHD in murine hematopoietic cell transplantation (HCT) models. Here, we tested 14 patients (age, 23-65 years), 12 with acute leukemia and 2 with myelodysplastic syndrome, who underwent HLA-mismatched HCT and subsequently received donor NK cell infusions. Cell donors (age, 16-51 years), comprising seven siblings, five offspring, and two mothers of the patients, underwent growth factor-mobilized leukapheresis for 3-5 days. Cells collected on the first 2-4 days were used for HCT, whereas those collected on the last day were CD34 selected by magnetic-activated cell sorting (median, 2.22 x 10(6) cells/kg; range, 0.29-5.66). Donor NK cells were generated from the CD34(+) cells by ex vivo cell culture over a 6-week period (median, 9.28 x 10(6) cells/kg; range, 0.33-24.50; CD122/CD56(+) 64%; CD3(+) 1.0%; and viability 88%). There were no signs of acute toxicity in patients infused with these cells 6-7 weeks post transplant. Overall, one and five patients developed acute and chronic GVHD during post transplant period, respectively. These results showed that clinical-grade donor NK cell production from CD34(+) cells is feasible. |
| Databáze: | OpenAIRE |
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