Molecular cloning of the mouse CCK gene: expression in different brain regions and during cortical development
| Autor: | A. Vashishtha, Maria Vitale, E. Linzer, D.J. Powell, Jeffrey M. Friedman |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
RNA Splicing
Molecular Sequence Data Restriction Mapping Glutamate decarboxylase Gene Expression Biology Mice Exon Sequence Homology Nucleic Acid Gene expression Genetics medicine Animals Humans Amino Acid Sequence Cloning Molecular Intestinal Mucosa Promoter Regions Genetic Gene Cholecystokinin Cerebral Cortex Genomic Library Base Sequence digestive oral and skin physiology Brain RNA DNA Exons Molecular biology Intestines medicine.anatomical_structure Cerebral cortex RNA splicing hormones hormone substitutes and hormone antagonists |
| Zdroj: | Nucleic Acids Research. 19:169-177 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/19.1.169 |
| Popis: | In this paper we describe experiments that address specific issues concerning the regulation of the mouse cholecystokinin gene in brain and intestine. The mouse cholecystokinin gene was cloned and sequenced. Extensive homology among the mouse, man and rat genes was noted particularly in the three exons and the regions upstream of the RNA start site. RNAse protection assays for each of the three exons were used to demonstrate that CCK is expressed in only a subset of tissues and that the same cap site and splice choices are used in brain, intestine as well as in cerebellum, cortex, midbrain, hypothalamus and hippocampus. CCK RNA was also noted to be detectable in kidney. Thus the same gene using the same promoter is expressed in subsets of cells that differ in their biochemical, morphologic and functional characteristics. The level of expression of CCK was also monitored during mouse cortical development and the appearance of CCK RNA was compared to glutamate decarboxylase (GAD), enkephalin and somatostatin. It was noted that each of these cortical markers was first expressed at different times during cortical development. The appearance of CCK RNA during intestinal development was also measured and found to precede appearance in cortex by several days. |
| Databáze: | OpenAIRE |
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