3-nitrotyrosine levels in dichlorvos-induced neurotoxicity
| Autor: | Abdurrahman Aksoy, Oguzhan Yavuz, Enes Atmaca, Dilek Guvenc, Yavuz Kursad |
|---|---|
| Přispěvatelé: | OMÜ |
| Rok vydání: | 2014 |
| Předmět: |
Male
Insecticides medicine.medical_specialty brain medicine.medical_treatment Toxicology High-performance liquid chromatography Lethal Dose 50 Rats Sprague-Dawley Random Allocation chemistry.chemical_compound Internal medicine Dichlorvos medicine Animals Tyrosine Saline Public Health Environmental and Occupational Health Neurotoxicity Brain pesticides medicine.disease Rats rats Oxidative Stress 3-nitrotyrosine Endocrinology chemistry Anesthesia Toxicity Forebrain Neurotoxicity Syndromes HPLC nitro-oxidative stress |
| Zdroj: | Archives of Industrial Hygiene and Toxicology. 65:109-112 |
| ISSN: | 0004-1254 |
| DOI: | 10.2478/10004-1254-65-2014-2416 |
| Popis: | Atmaca, Enes/0000-0002-8978-3755; Aksoy, Abdurrahman/0000-0001-9486-312X WOS: 000333849700011 PubMed: 24535296 The aim of this study was to evaluate dichlorvos toxicity in terms of nitro-oxidative stress by determining 3-nitrotyrosine (3-NT) levels in the fore, mid, and hindbrain regions in acutely exposed rats. Male Sprague-Dawley rats were randomly allocated to three groups of eight. Group 1 was administered a single intraperitoneal dichlorvos dose of 1.8 mg kg(-1) (0.1xLD(50)) and group 2 a dose of 9 mg kg(-1) (0.5xLD(50)). The control group received 0.5 mL saline solution via the same route. 3-NT and tyrosine (TYR) levels were measured using high performance liquid chromatography with a photodiode array detector (HPLC-PDA) and expressed as a ratio of 3-NT to TYR. The 3-NT/1000 TYR ratios increased significantly in the fore-, mid- and hindbrains of the exposed groups compared to control (p < 0.01). In the forebrain, the increase was also significant between the treated groups. Our study has confirmed that acute exposure to dichlorvos leads to nitro-oxidative stress in the brain and that 3-NT may play a role in the mechanism of dichlorvos neurotoxicity. Commission of Scientific Research Projects of Ondokuz Mayis UniversityOndokuz Mayis University [PYO.VET.1901.10.002] This study was supported by the Commission of Scientific Research Projects of Ondokuz Mayis University (Project No: PYO.VET.1901.10.002). The authors wish to thank Gregory T. Sullivan for editing the English in this manuscript. |
| Databáze: | OpenAIRE |
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