Evaluation of two molecular methods for the detection of Yellow fever virus genome
| Autor: | W. Ian Lipkin, Pedro Fernando da Costa Vasconcelos, Nazir Savji, Márcio Roberto Teixeira Nunes, Keley N. B Nunes, Lívia Carício Martins, Samir Mansour Moraes Casseb, Juarez Antônio Simões Quaresma, Gustavo Palacios |
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| Rok vydání: | 2011 |
| Předmět: |
Serum
Sa?de P?blica Biology Febre Amarela / transmiss?o Genome Virus Article law.invention Flaviviridae Arbovirus / isolamento & purifica??o law Cricetinae Virology V?rus da Febre Amarela Yellow Fever medicine V?rus da Febre Amarela / isolamento & purifica??o Animals Humans Polymerase chain reaction Mesocricetus Reverse Transcriptase Polymerase Chain Reaction Yellow fever Outbreak virus diseases South America biology.organism_classification medicine.disease Flavivirus Molecular Diagnostic Techniques Febre Amarela / epidemiologia Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos Yellow fever virus Nested polymerase chain reaction |
| Zdroj: | Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| ISSN: | 1879-0984 |
| Popis: | Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Columbia University. Mailman School of Public Health. Center for Infection and Immunity. New York, NY, USA. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Par? State University. Department of Pathology. Bel?m, PA, Brazil. Columbia University. Mailman School of Public Health. Center for Infection and Immunity. New York, NY, USA. Columbia University. Mailman School of Public Health. Center for Infection and Immunity. New York, NY, USA. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Par? State University. Department of Pathology. Bel?m, PA, Brazil. Yellow fever virus (YFV), a member of the family Flaviviridae, genus Flavivirus is endemic to tropical areas of Africa and South America and is among the arboviruses that pose a threat to public health. Recent outbreaks in Brazil, Bolivia, and Paraguay and the observation that vectors capable of transmitting YFV are presenting in urban areas underscore the urgency of improving surveillance and diagnostic methods. Two novel methods (RT-hemi-nested-PCR and SYBR(?) Green qRT-PCR) for efficient detection of YFV strains circulating in South America have been developed. The methods were validated using samples obtained from golden hamsters infected experimentally with wild-type YFV strains as well as human serum and tissue samples with acute disease. |
| Databáze: | OpenAIRE |
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