Cell-Surface Interactions: Differential Inhibition by Proflavine of Embryonic Cell Aggregation and Production of Specific Cell-Aggregating Factor
| Autor: | R. E. Hausman, A. A. Moscona |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1973 |
| Předmět: |
Cell
Cell Separation Chick Embryo Biology Diamines Tritium Retina chemistry.chemical_compound Lectins medicine Concanavalin A Animals Carbon Radioisotopes Amino Acids Biological Sciences: Cell Biology Uridine Proflavine Cells Cultured Cell Aggregation chemistry.chemical_classification Glucosamine Multidisciplinary Proteins Embryonic stem cell Cell aggregation Amino acid Cell biology Molecular Weight medicine.anatomical_structure chemistry Biochemistry biology.protein Acridines Electrophoresis Polyacrylamide Gel Molecular probe |
| Popis: | Cell recognition and morphogenetic aggregation of embryonic cells into tissues are mediated by specific macromolecules in the cell surface (cell-aggregating factors). A factor specific for embryonic neural retina cells was demonstrated; its synthesis is required for histotypic reaggregation of retina cell suspensions. We show that proflavine (3,6-diaminoacridine) preferentially and reversibly suppresses production of the cell-aggregating factor and thereby inhibits normal cell reaggregation. If such proflavine-treated retina cells are exogenously supplied with the retina-specific factor, they reaggregate. The selectivity of these effects supports the postulated significance of specific cell-surface components in cell association into tissues; the results indicate that proflavine may be a useful molecular probe for studying formation of specific cell-surface components and their role in various cell interactions. |
| Databáze: | OpenAIRE |
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