Expression of suppressor of cytokine signaling 1 and 3 in ligature-induced periodontitis in rats

Autor: Carlos Rossa Jr, J. Paulo Vilas-Boas, Joni Cirelli, Andressa V. B. Nogueira, Joao Antonio Chaves de Souza, Pedro Paulo Chaves de Souza, Gustavo Garlet
Přispěvatelé: Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP)
Jazyk: angličtina
Předmět:
Male
Time Factors
medicine.medical_treatment
Alveolar Bone Loss
Gingiva
Signal transduction
Image Processing
Computer-Assisted
SOCS3
STAT3
biology
medicine.diagnostic_test
General Medicine
Interleukin-10
STAT1 Transcription Factor
Connective Tissue
Collagen
medicine.symptom
Periodontal disease
STAT3 Transcription Factor
medicine.medical_specialty
Inflammation
CITOCINAS
Suppressor of Cytokine Signaling 1 Protein
Western blot
Internal medicine
medicine
Animals
Rats
Wistar
STAT transcription factors
Ligature
Periodontitis
General Dentistry
Dental alveolus
Suppressor of cytokine signaling 1
Dentistry(all)
Interleukin-6
Tumor Necrosis Factor-alpha
RANK Ligand
Osteoprotegerin
Cell Biology
Fibroblasts
medicine.disease
Rats
Disease Models
Animal
Endocrinology
Otorhinolaryngology
Suppressor of Cytokine Signaling 3 Protein
Suppressor of cytokine signaling proteins
Janus kinases
biology.protein
Zdroj: Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
ISSN: 0003-9969
DOI: 10.1016/j.archoralbio.2011.03.022
Popis: Made available in DSpace on 2013-09-27T14:54:21Z (GMT). No. of bitstreams: 1 WOS000296112200025.pdf: 1098345 bytes, checksum: c50cb919029b6aa059f1ad31044dd928 (MD5) Previous issue date: 2011-10-01 Made available in DSpace on 2013-09-30T18:32:47Z (GMT). No. of bitstreams: 1 WOS000296112200025.pdf: 1098345 bytes, checksum: c50cb919029b6aa059f1ad31044dd928 (MD5) Previous issue date: 2011-10-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:45:15Z No. of bitstreams: 1 WOS000296112200025.pdf: 1098345 bytes, checksum: c50cb919029b6aa059f1ad31044dd928 (MD5) Made available in DSpace on 2014-05-20T13:45:15Z (GMT). No. of bitstreams: 1 WOS000296112200025.pdf: 1098345 bytes, checksum: c50cb919029b6aa059f1ad31044dd928 (MD5) Previous issue date: 2011-10-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) State of São Paulo, Brazil Objective: : Evaluate expression of inducible negative regulators of JAK/STAT pathway and their target proteins during the course of ligature-induced experimental periodontal disease in rats.Design: : Rats were sacrificed 07, 15 and 30 days after disease induction for histological evaluation of periodontal inflammation and macroscopic analysis of alveolar bone loss. SOCS expression and the activation status of STAT1 and STAT3 were evaluated in gingival biopsies by real time PCR and Western blot.Results: : Ligature-induced model presented significant progressive bone loss from 7 to 30 days. Inflammation was evident and similar for 07 and 15 days; however, a decrease on severity at the end of the experimental period was observed. There was a significant (p < 0.05) increase on SOCS1 and SOCS3 gene expression in PD compared to control group at 15 and 30 days. The SOCS1 and SOCS3 protein expression and activation of STAT1 and STAT3 were increased in earlier periods in the ligature model.Conclusion: : This study suggests that SOCS1 and SOCS3 were directly correlated with regulatory mechanism of the inflammatory process responsible for the periodontal disease destruction. (C) 2011 Elsevier Ltd. All rights reserved. Univ Estadual Paulista UNESP, Sch Dent Araraquara, Dept Diag & Surg, BR-14801903 Araraquara, SP, Brazil USP, Sch Dent Bauru, Dept Biol Sci, BR-17012901 Bauru, SP, Brazil Univ Estadual Paulista UNESP, Sch Dent Araraquara, Dept Physiol & Pathol, BR-14801903 Araraquara, SP, Brazil Univ Estadual Paulista UNESP, Sch Dent Araraquara, Dept Diag & Surg, BR-14801903 Araraquara, SP, Brazil Univ Estadual Paulista UNESP, Sch Dent Araraquara, Dept Physiol & Pathol, BR-14801903 Araraquara, SP, Brazil FAPESP: 07/06658-7 FAPESP: 07/06332-4 State of São Paulo, Brazil: 2007/06658-7 State of São Paulo, Brazil: 2007/06332-4
Databáze: OpenAIRE
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