Bovine intelectin 2 expression as a biomarker of paratuberculosis disease progression
| Autor: | Ramón A. Juste, Ana Balseiro, Cristina Blanco Vázquez, Rosa Casais, Natalia Iglesias, Marta Alonso-Hearn, Maria Canive |
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| Přispěvatelé: | CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Balseiro Morales, Ana María, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España), Balseiro Morales, Ana María [0000-0002-5121-7264] |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Ileocecal valve
Pathology medicine.medical_specialty 040301 veterinary sciences Veterinary medicine Paratuberculosis macromolecular substances Biology environment and public health Article Enteritis 0403 veterinary science 03 medical and health sciences ileocecal valve intelectin 2 SF600-1100 medicine 030304 developmental biology 0303 health sciences Innate immune system General Veterinary integumentary system Mycobacterium avium subsp. paratuberculosis Disease progression Biomarker 04 agricultural and veterinary sciences medicine.disease Immunohistochemistry medicine.anatomical_structure paratuberculosis QL1-991 immunohistochemistry Biomarker (medicine) Intelectin 2 biomarker Animal Science and Zoology Zoology Biomarkers |
| Zdroj: | Animals Volume 11 Issue 5 Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) Animals : an Open Access Journal from MDPI Animals, Vol 11, Iss 1370, p 1370 (2021) Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 13 páginas, 3 tablas, 2 figuras. Paratuberculosis (PTB), a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (MAP), is responsible for important economic losses in the dairy industry. Our previous RNA-sequencing (RNA-Seq) analysis showed that bovine intelectin 2 (ITLN2) precursor gene was overexpressed in ileocecal valve (ICV) samples of animals with focal (log2 fold-change = 10.6) and diffuse (log2 fold-change = 6.8) PTB-associated lesions compared to animals without lesions. This study analyzes the potential use of ITLN2, a protein that has been described as fundamental in the innate immune response to infections, as a biomarker of MAP infection. The presence of ITLN2 was investigated by quantitative immunohistochemical analysis of ICV samples of 20 Holstein Friesian cows showing focal (n = 5), multifocal (n = 5), diffuse (n = 5) and no histological lesions (n = 5). Significant differences were observed in the mean number of ITLN2 immunostained goblet and Paneth cells between the three histopathological types and the control. The number of immunolabelled cells was higher in the focal histopathological type (116.9 ± 113.9) followed by the multifocal (108.7 ± 140.5), diffuse (76.5 ± 97.8) and control types (41.0 ± 81.3). These results validate ITLN2 as a post-mortem biomarker of disease progression. This work has been funded by the National Institute for Agricultural Research (INIA RTA2014-00009-C02), the Ministerio de Ciencia, Innovación y Universidades (MCIU) and the Agencia Estatal de Investigación (AEI) reference project RTI2018-094192-R-C22 (FEDER co-funded). Cristina Blanco Vázquez and Maria Canive were supported by a grant from Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA, Spain). |
| Databáze: | OpenAIRE |
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