Brush Border Particulates of Renal Tissue
| Autor: | Elizabeth Milikin, Francis Binkley, Reba K. Wright, Ina Jane Wundram, Neva King, C. H. O'Neal |
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| Rok vydání: | 1968 |
| Předmět: |
Brush border
Phospholipid Surface-Active Agents chemistry.chemical_compound Adenosine Triphosphate Leucine Animals Centrifugation Pyrophosphatases Adenosine Triphosphatases chemistry.chemical_classification Carbon Isotopes Membranes Multidisciplinary Alkaline Phosphatase Molecular biology Centrifugation Zonal Rats Amino acid Microscopy Electron Kidney Tubules chemistry Biochemistry Puromycin Glucose-6-Phosphatase Alkaline phosphatase Ribosomes Adenosine triphosphate Peptide Hydrolases |
| Zdroj: | Science. 162:1009-1011 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.162.3857.1009 |
| Popis: | Particulates containing a large part of the alkaline phosphatase activity of renal tissue were separated from homogenates and from ribosomal preparations by zonal centrifugation. The particles had a high content of phospholipid and cholesterol that was not removed by treatment with I percent deoxycholate. Enzymatic activities concentrated with the particles were the alkaline phosphatase, a peptidase resistant to proteolysis, glucose-6-phosphatase, inorganic pyrophos-phatase, and adenosine triphosphatase. The particles accumulated leucine with no stimulation from soluble factors and with inhibition by other amino acids; the accumulation was stimulated by adenosine triphosphate and was not inhibited by puromycin. The particles appear to be derived from the membranes of the brush borders of tubular cells. |
| Databáze: | OpenAIRE |
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