An Inhibitory Effect of Chrysoeriol on Platelet-Derived Growth Factor (PDGF)-Induced Proliferation and PDGF Receptor Signaling in Human Aortic Smooth Muscle Cells
| Autor: | Takayuki Yonezawa, Byung-Yoon Cha, Kazuo Nagai, Toshiaki Teruya, Je-Tae Woo, Wen Lei Shi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Platelet-derived growth factor Cell Survival medicine.medical_treatment Blotting Western Myocytes Smooth Muscle Biology Pharmacology Chrysoeriol p38 Mitogen-Activated Protein Kinases chemistry.chemical_compound Cell Movement Internal medicine medicine Myocyte Humans Receptors Platelet-Derived Growth Factor Phosphorylation Receptor Extracellular Signal-Regulated MAP Kinases Cells Cultured Cytoskeleton Cell Proliferation Flavonoids Platelet-Derived Growth Factor Growth factor lcsh:RM1-950 Flavones Actins Oncogene Protein v-akt Endocrinology lcsh:Therapeutics. Pharmacology chemistry biology.protein Molecular Medicine Electrophoresis Polyacrylamide Gel Signal transduction Platelet-derived growth factor receptor Signal Transduction Thymidine |
| Zdroj: | Journal of Pharmacological Sciences, Vol 110, Iss 1, Pp 105-110 (2009) |
| ISSN: | 1347-8613 |
| Popis: | Platelet-derived growth factor (PDGF)-BB is one of the most potent factors in the development and progression of various vascular disorders such as restenosis and atherosclerosis. Chrysoeriol is a flavonoid with antioxidant and anti-inflammatory activities. In this study, we investigated the effect of chrysoeriol on the proliferation of human aortic smooth muscle cells (HASMC). Chrysoeriol significantly inhibited PDGF (20 ng/mL)-induced migration and [3H]-thymidine incorporation into DNA at concentrations of 5 and 10 μM without any cytotoxicity. Chrysoeriol also blocked PDGF-stimulated dissociation of actin filament and inhibited PDGF beta-receptor (Rβ) phosphorylation in a concentration-dependent manner. As a result, the downstream signal transduction pathways of PDGF-Rβ, including ERK1/2, p38, and Akt phosphorylation, were also inhibited by chrysoeriol in the same pattern. These findings suggest that in addition to its antioxidant and anti-inflammatory activities, chrysoeriol may be used for the prevention and treatment of vascular diseases and during restenosis after coronary angioplasty. Keywords:: smooth muscle cell, chrysoeriol, platelet-derived growth factor (PDGF)-induced proliferation, antiproliferative, migration |
| Databáze: | OpenAIRE |
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