Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers

Autor: Marino DiFranco, Julio L. Vergara
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Time Factors
Confocal
Muscle Fibers
Skeletal
chemistry.chemical_element
Skeletal Muscle Fibers
Calcium
General Biochemistry
Genetics and Molecular Biology
confocal spot detection
Membrane Potentials
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Membrane Microdomains
Animals
Muscle
Skeletal
lcsh:QH301-705.5
030304 developmental biology
caffeine
0303 health sciences
Rana catesbeiana
Chemistry
calcium transients
excitation-contraction coupling
General Medicine
Anatomy
Frog skeletal muscle
calcium microdomains
3. Good health
Coupling (electronics)
Electrophysiology
Microscopy
Fluorescence
lcsh:Biology (General)
frog skeletal muscle
Biophysics
Central Nervous System Stimulants
General Agricultural and Biological Sciences
Caffeine
030217 neurology & neurosurgery
Muscle Contraction
Zdroj: Biological Research, Vol 39, Iss 3, Pp 567-581 (2006)
Biological Research, Volume: 39, Issue: 3, Pages: 567-581, Published: 2006
Biological Research v.39 n.3 2006
SciELO Chile
CONICYT Chile
instacron:CONICYT
ISSN: 0717-6287
0716-9760
Popis: The effects of caffeine on the process of excitation-contraction coupling in amphibian skeletal muscle fibers were investigated using the confocal spot detection technique. This method permits to carefully discriminate between caffeine effects on the primary sources of Ca2+ release at the Z-lines where the triads are located and secondary actions on other potential Ca Release sources. Our results demonstrate that 0.5 mM caffeine potentiates and prolongs localized action-potential evoked Ca2+ transients recorded at the level of the Z-lines, but that 1mM only prolongs them. The effects at both doses are reversible. At the level of the M-line, localized Ca2+ transients displayed more variability in the presence of 1 mM caffeine than in control conditions. At this dose of caffeine, extra-junctional sources of Ca2+ release also were observed occasionally.
Databáze: OpenAIRE
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