Complex effect of continuous curcumin exposure on human bone marrow‐derived mesenchymal stem cell regenerative properties through matrix metalloproteinase regulation
| Autor: | Xiang‐Ling Yuan, Samantha Antonio Leong, Qichen Yang, Kwok Ping Chan, Tsz Kin Ng |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Curcumin
MMP1 Apoptosis Pharmacology Matrix metalloproteinase Toxicology 030226 pharmacology & pharmacy Neuroprotection 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Movement Osteogenesis Matrix Metalloproteinase 13 Matrix Metalloproteinases Secreted Humans Indoleamine-Pyrrole 2 3 -Dioxygenase Regeneration Cells Cultured Cell Proliferation Adipogenesis Mesenchymal stem cell Mesenchymal Stem Cells General Medicine chemistry Toxicity Matrix Metalloproteinase 1 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Basic & Clinical Pharmacology & Toxicology. 128:141-153 |
| ISSN: | 1742-7843 1742-7835 |
| DOI: | 10.1111/bcpt.13477 |
| Popis: | Curcumin has been reported to be beneficial for cancers, cardiovascular and neurodegenerative diseases, based on its anti-oxidative, anti-inflammation, anti-tumorigenic and neuroprotective properties. With its high-dose application, curcumin toxicity to systemic tissues is a reasonable concern. Here, we report the responses of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) to continuous curcumin exposure. hBM-MSCs were treated with 0.01-100 μmol/L curcumin continuously in vitro for 7 days. 25 μmol/L curcumin or above significantly attenuated hBM-MSC maintenance, whereas 10 μmol/L curcumin reduced hBM-MSC proliferation and hindered their migration with increasing cell apoptosis. Besides, 5 μmol/L curcumin treatment inhibited hBM-MSC adipogenic differentiation, but enhanced osteogenic differentiation, which depended on matrix metalloproteinase (MMP)-13 expression and activity. Furthermore, curcumin treatment reduced MMP1 expression but up-regulated the immunomodulatory gene IDO1 expression. In summary, this study revealed the complex effects of continuous curcumin exposure on hBM-MSC maintenance and regenerative properties through MMP regulation. Given the complex effects of curcumin, its use for biomedical purposes should be carefully considered in treatment length and dosage. |
| Databáze: | OpenAIRE |
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