Detection of spatial localization of Hst-1/Fgf-4 gene expression in brain and testis from adult mice
| Autor: | Takahiro Ochiya, Noriko Osumi, Masaaki Terada, Yasuo Ishii, Hiromi Sakamoto, Hanako Yamamoto, Yasushi Takahama |
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| Rok vydání: | 2000 |
| Předmět: |
Male
Cancer Research Cerebellum Fibroblast Growth Factor 4 Gene Expression In situ hybridization Biology Fibroblast growth factor Mice FGF9 Proto-Oncogene Proteins Testis Gene expression Genetics medicine Animals Tissue Distribution RNA Messenger Molecular Biology Gene Cells Cultured In Situ Hybridization Mice Inbred ICR Brain 3T3 Cells Sertoli cell Molecular biology Fibroblast Growth Factors Mice Inbred C57BL Real-time polymerase chain reaction medicine.anatomical_structure Female |
| Zdroj: | Oncogene. 19:3805-3810 |
| ISSN: | 1476-5594 0950-9232 |
| DOI: | 10.1038/sj.onc.1203752 |
| Popis: | HST-1, a member of the fibroblast growth factor (FGF) family (FGF-4), has been shown to be a signaling molecule whose expression is essential for embryonic development. However, HST-1/FGF-4 expression has not been detected or reported in adult tissues so far analysed. To investigate whether there is a possible role of HST-1/FGF-4 in adult stage, we have carried out a highly sensitive RT-PCR analysis of Hst-1/Fgf-4 gene expression in adult mice tissues. Results show Hst-1/Fgf-4 gene expression in the nervous system, intestines, and testis of normal adult mice. In situ hybridization technique was used to localize Hst-1/Fgf-4 gene expression in the cerebellum and testis from 10-week-old mice. Cell type-specific gene expression was detected: Purkinje cells in the cerebellum and Sertoli cells in testis. These findings suggest that the Hst-1/Fgf-4 gene also plays an important role in adult tissues, and may offer insights into the biological significance of HST-1/FGF-4 in cerebellar and testicular functions. |
| Databáze: | OpenAIRE |
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