Quasispecies variation of porcine reproductive and respiratory syndrome virus during natural infection☆
| Autor: | Suzanne M Milburn, James Lowe, Lawrence D. Firkins, Tony L. Goldberg |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Nonsynonymous substitution
PRRS virus Swine Evolution animal diseases Natural selection Molecular Sequence Data Palatine Tonsil Porcine Reproductive and Respiratory Syndrome Variation Viral quasispecies Arteriviridae Structural variation Viral Envelope Proteins Virology Genetic variation Genotype Animals Porcine respiratory and reproductive syndrome virus Genetic variability Cloning Molecular Gene Phylogeny Genetics biology Genetic Variation Sequence Analysis DNA Porcine reproductive and respiratory syndrome virus biology.organism_classification Quasispecies Blood RNA Viral Female |
| Zdroj: | Virology. (2):197-207 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2003.07.009 |
| Popis: | Porcine reproductive and respiratory syndrome virus (PRRSV) displays notorious genetic, antigenic, and clinical variability. Little is known, however, about the nature and extent of viral variation present within naturally infected animals. By amplifying and cloning the open reading frame 5 gene from tonsils of naturally infected swine, and by sequencing individual clones, we characterized viral diversity in nine animals from two farms. All animals harbored multiple PRRSV variants at both the nucleic and the amino acid levels. Structural variation and rates of synonymous and nonsynonymous nucleotide substitution were no different within known epitopes than elsewhere. Analysis of molecular variance indicated that differences between farms, among animals within farms, and within individual animals accounted for 92.94, 3.84, and 3.22% of the total viral genetic variability observed, respectively. PRRSV exists during natural infection as a quasispecies distribution of related genotypes. Positive natural selection for immune evasiveness does not appear to maintain this diversity. |
| Databáze: | OpenAIRE |
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