Transforming growth factor-alpha, epidermal growth factor, and epidermal growth factor receptor in human testis obtained from biopsy and castration: immunohistochemical study
| Autor: | Ikuo Maehara, Hironobu Sasano, Hisayoshi Nakazumi, Seiichi Orikasa |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Male endocrine system medicine.medical_specialty TGF alpha Adolescent General Biochemistry Genetics and Molecular Biology Testicular Neoplasms Epidermal growth factor Internal medicine Testis medicine Humans Epidermal growth factor receptor Autocrine signalling Spermatogenesis Infertility Male Aged Aged 80 and over biology Epidermal Growth Factor urogenital system Prostatic Neoplasms General Medicine Middle Aged Transforming Growth Factor alpha Sertoli cell Immunohistochemistry ErbB Receptors Endocrinology medicine.anatomical_structure Transforming growth factor beta 3 biology.protein A431 cells Orchiectomy Transforming growth factor |
| Zdroj: | The Tohoku journal of experimental medicine. 178(4) |
| ISSN: | 0040-8727 |
| Popis: | We analyzed expression of transforming growth factor (TGF)-alpha, epidermal growth factor (EGF) and their receptor, EGF receptor (EGFR), by immunohistochemistry in the human testis to determine the possible roles of these growth factors in human testicular function. Specimens were obtained from 17 patients including 9 patients with infertility, 4 patients with prostatic carcinoma and 4 patients with contralateral testicular tumor. EGF immunoreactivity was positive in the hyperplasic Leydig cells of one patient but negative in the other cases. On the other hand, strong TGF-alpha immunoreactivity was observed in Leydig cells, with weak staining in Sertoli cells and germ cells in cases with normal spermatogenesis. EGFR immunoreactivity was observed in the Leydig and peritubular cells, appearing as membrane staining. Marked immunoreactivity for TGF-alpha was observed in the Sertoli cells in testes with decreased spermatogenesis, especially in the Sertoli-cell-only syndrome. This finding may indicate a compensatory increase of TGF-alpha expression in the Sertoli cells accompanying a decrease in spermatogenesis. No significant correlation was found between the degrees of spermatogenesis and immunolocalization of the EGF receptor. These findings suggest that TGF-alpha is a locally produced growth factor that is involved in spermatogenesis in the human testis via an autocrine and/or paracrine mechanism. |
| Databáze: | OpenAIRE |
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