Quantitative Analysis of Rutin by HPTLC and In Vitro Antioxidant and Antibacterial Activities of Phenolic-Rich Extracts from Verbesina sphaerocephala
| Autor: | Rafael Salgado-Garciglia, Monserrat Vázquez-Sánchez, Luis Fernando Ceja-Torres, Dioselina Álvarez-Bernal, Kathia Yanelly Rodríguez-Valdovinos, José Roberto Medina-Medrano, Ernesto Oregel-Zamudio |
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| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
Verbesina DPPH medicine.medical_treatment Flavonoid phenolic compounds Plant Science 01 natural sciences Article 03 medical and health sciences Rutin chemistry.chemical_compound scavenging activity medicine Ecology Evolution Behavior and Systematics 030304 developmental biology chemistry.chemical_classification 0303 health sciences antimicrobial activity Ecology biology Traditional medicine rutin 010401 analytical chemistry Botany Antimicrobial biology.organism_classification Ascorbic acid 0104 chemical sciences chemistry QK1-989 Composition (visual arts) high-performance thin-layer chromatography (HPTLC) |
| Zdroj: | Plants Volume 10 Issue 3 Plants, Vol 10, Iss 475, p 475 (2021) |
| ISSN: | 2223-7747 |
| Popis: | Verbesina sphaerocephala A. Gray, like other wild plants of the genus Verbesina, has been used in herbal medicine. There is information for other species of the genus related to their phenolic content, antioxidant capacity, and isolation of bioactive compounds with antimicrobial activity. However, there are no reports for V. sphaerocephala, although it has an important presence in the state of Michoacán, México. In this study, the phenolic composition, quantification of rutin, and in vitro antioxidant and antibacterial activities of methanolic extracts from V. sphaerocephala leaves and flowers were determined. The results showed that all the investigated extracts have high phenolic and flavonoid contents. The flavonoid rutin was identified in all the extracts from V. sphaerocephala by high-performance thin-layer chromatography (HPTLC). The V. sphaerocephala extracts showed scavenging activity against DPPH• and ABTS•+ radicals (IC50 and 5.83 ± 0.50 and 0.93 ± 0.01 mg/mL, respectively) as well as relevant antioxidant capacity (51.05 ± 0.36 mg of ascorbic acid/g of dry tissue). The experimental results show that V. sphaerocephala extracts possessed a strong antimicrobial activity against Escherichia coli and Staphylococcus aureus bacteria. This research indicates that V. sphaerocephala could be considered as a potential source of natural compounds from the point of ethnopharmacological usage. |
| Databáze: | OpenAIRE |
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