The budding yeast RSC complex maintains ploidy by promoting spindle pole body insertion
| Autor: | Jiongwen Ou, Melainia McClain, Jay R. Unruh, Godai Suzuki, Won-Ki Huh, Sue L. Jaspersen, Zulin Yu, Tina L. Sing, Jesse Marshall-Sheppard, Michael Costanzo, Yoshikazu Ohya, Shinsuke Ohnuki, Bryan-Joseph San Luis, Charles Boone, Minnie P. Hung, Grant W. Brown |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Proteins Chromosomal Proteins Non-Histone Nuclear Envelope Saccharomyces cerevisiae Cell Cycle Proteins macromolecular substances Spindle Apparatus Spindle pole body Article Chromosome segregation 03 medical and health sciences Chromosome Segregation RSC complex Chromatin structure remodeling (RSC) complex Research Articles Ploidies biology Nuclear Proteins Cell Biology biology.organism_classification Chromatin Cell biology DNA-Binding Proteins Nuclear Pore Complex Proteins Cytoskeletal Proteins 030104 developmental biology Nondisjunction Spindle Pole Bodies biology.protein Ploidy Transcription Factors |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Sing et al. characterize an unanticipated role for the Saccharomyces cerevisiae RSC complex in ploidy maintenance. They show that RSC promotes the distribution of Nbp1 and Ndc1 to the spindle pole body (SPB) to facilitate SPB maturation and accurate chromosome segregation. Ploidy is tightly regulated in eukaryotic cells and is critical for cell function and survival. Cells coordinate multiple pathways to ensure replicated DNA is segregated accurately to prevent abnormal changes in chromosome number. In this study, we characterize an unanticipated role for the Saccharomyces cerevisiae “remodels the structure of chromatin” (RSC) complex in ploidy maintenance. We show that deletion of any of six nonessential RSC genes causes a rapid transition from haploid to diploid DNA content because of nondisjunction events. Diploidization is accompanied by diagnostic changes in cell morphology and is stably maintained without further ploidy increases. We find that RSC promotes chromosome segregation by facilitating spindle pole body (SPB) duplication. More specifically, RSC plays a role in distributing two SPB insertion factors, Nbp1 and Ndc1, to the new SPB. Thus, we provide insight into a role for a SWI/SNF family complex in SPB duplication and ploidy maintenance. |
| Databáze: | OpenAIRE |
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