Development of an attenuated strain of chikungunya virus for use in vaccine production
| Autor: | Helen H. Ramsburg, Neil H. Levitt, Harold W. Lupton, Sherman E. Hasty, Francis E. Cole, Patricia M. Repik |
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| Rok vydání: | 1986 |
| Předmět: |
Clone (cell biology)
Oligonucleotides medicine.disease_cause Antibodies Viral Vaccines Attenuated Virus Neutralization Mice Togaviridae Infections Cytopathogenic Effect Viral medicine Animals Humans Chikungunya Neutralizing antibody Mice Inbred ICR Attenuated vaccine General Veterinary General Immunology and Microbiology biology Viral Vaccine Public Health Environmental and Occupational Health Temperature Viral Vaccines Virology Macaca mulatta Animals Suckling Infectious Diseases biology.protein Molecular Medicine RNA Viral Antibody Chikungunya virus |
| Zdroj: | Vaccine. 4(3) |
| ISSN: | 0264-410X |
| Popis: | An attenuated chikungunya (CHIK) virus clone was developed for production of a live vaccine for human use. CHIK strain 15561 was subjected to 18 plaque-to-plaque passages in MRC-5 cultures before CHIK 181/clone 25 was selected as vaccine seed based on homogeneous small plaque size, suckling mouse avirulence, reduced monkey viraemia and genetic stability. Oligonucleotide mapping demonstrated differences between parent and clone. Vaccine (pilot-lot production) elicited neutralizing antibody and protected mice and rhesus monkeys against challenge. After challenge, viraemias were absent in vaccinated monkeys. Vaccine was then produced and tested in accordance with governmental regulatory requirements of human use. |
| Databáze: | OpenAIRE |
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