Two Intercalation Mechanisms of Oxazole Yellow Dimer (YOYO-1) into DNA
| Autor: | Marta Pilz, Robert Hołyst, Karolina Kucharska, Krzysztof Bielec, Patrycja Kuźma, Tomasz Kalwarczyk |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Base pair
Dimer Intercalation (chemistry) Pharmaceutical Science Organic chemistry Article Analytical Chemistry chemistry.chemical_compound equilibrium constant QD241-441 intercalation Drug Discovery Molecule Physical and Theoretical Chemistry Binding site Equilibrium constant Fluorescent Dyes YOYO-1 Benzoxazoles Quinolinium Compounds DNA Intercalating Agents Crystallography chemistry Chemistry (miscellaneous) Quinolines brightness method Molecular Medicine Dimerization |
| Zdroj: | Molecules Volume 26 Issue 12 Molecules, Vol 26, Iss 3748, p 3748 (2021) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules26123748 |
| Popis: | The oxazole yellow dye, YOYO-1 (a symmetric homodimer), is a commonly used molecule for staining DNA. We applied the brightness analysis to study the intercalation of YOYO-1 into the DNA. We distinguished two binding modes of the dye to dsDNA: mono-intercalation and bis-intercalation. Bis-intercalation consists of two consecutive mono-intercalation steps, characterised by two distinct equilibrium constants (with the average number of base pair per binding site equals 3.5): K1=3.36±0.43×107M−1 and K2=1.90±0.61×105M−1, respectively. Mono-intercalation dominates at high concentrations of YOYO-1. Bis-intercalation occurs at low concentrations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |