Microtubule-associated protein 2 mediates induction of long-term potentiation in hippocampal neurons
| Autor: | Seulgi Noh, Ryan Bellmore, You-Na Jang, Ji Young Mun, Bridget N. Queenan, Kea Joo Lee, Hyungju Park, Nari Kim, Yoonju Kim, Hye-Yeon Kim, Ji-Young Kim, Jong-Cheol Rah, Daniel T.S. Pak |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Dendritic spine MAP Kinase Signaling System Dendritic Spines Long-Term Potentiation Stimulation AMPA receptor Hippocampal formation In Vitro Techniques Biochemistry 03 medical and health sciences 0302 clinical medicine Genetics medicine Animals Receptors AMPA RNA Small Interfering Microscopy Immunoelectron Molecular Biology CA1 Region Hippocampal Cells Cultured Neuronal Plasticity Chemistry musculoskeletal neural and ocular physiology Pyramidal Cells Long-term potentiation Cell biology Rats Mice Inbred C57BL Protein Transport 030104 developmental biology medicine.anatomical_structure nervous system Schaffer collateral Synaptic plasticity NMDA receptor RNA Interference Microtubule-Associated Proteins 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES. 34(5) |
| ISSN: | 1530-6860 |
| Popis: | Microtubule-associated protein (MAP) 2 has been perceived as a static cytoskeletal protein enriched in neuronal dendritic shafts. Emerging evidence indicates dynamic functions for various MAPs in activity-dependent synaptic plasticity. However, it is unclear how MAP2 is associated with synaptic plasticity mechanisms. Here, we demonstrate that specific silencing of high-molecular-weight MAP2 in vivo abolished induction of long-term potentiation (LTP) in the Schaffer collateral pathway of CA1 pyramidal neurons and in vitro blocked LTP-induced surface delivery of AMPA receptors and spine enlargement. In mature hippocampal neurons, we observed rapid translocation of a subpopulation of MAP2, present in dendritic shafts, to spines following LTP stimulation. Time-lapse confocal imaging showed that spine translocation of MAP2 was coupled with LTP-induced spine enlargement. Consistently, immunogold electron microscopy revealed that LTP stimulation of the Schaffer collateral pathway promoted MAP2 labeling in spine heads of CA1 neurons. This translocation depended on NMDA receptor activation and Ras-MAPK signaling. Furthermore, LTP stimulation led to an increase in surface-expressed AMPA receptors specifically in the neurons with MAP2 spine translocation. Altogether, this study indicates a novel role for MAP2 in LTP mechanisms and suggests that MAP2 participates in activity-dependent synaptic plasticity in mature hippocampal networks. |
| Databáze: | OpenAIRE |
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